Kubke Lab:Research/CND/Records/NF001: Difference between revisions

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'''NF001 - embryo sb - Stage 20-21'''
'''NF001 - embryo sb - Stage 20-21 - 2006.08.02'''
2/8/06


==Experiment==
'''Objective'''
Try to differentiate between rostral and caudal components of cochleovestibular ganglion
'''Procedure'''
Both dyes inkected on LEFT.
* DiI crystals (anterior VIII) 
* DiO crystals (posterior VIII)
'''Notes:'''
Some splatter.
Rhombomere boundaries visible and clear
Rhombomere boundaries visible and clear
DiI (anterior VIII) and DiO (posterior VIII)
Crystals
Some splatter.


'''Results'''
==Files==
DiI: MNs on both ipsi and contra side of brainstem
* nf001 series 012 ch00 z00-81 - Overexposed. Splatter visible
DiO: no uptake
 
==Results==
'''anterior VIII'''
MNs on both ipsi and contra side of brainstem
 
'''posterior VIII'''
No labelling


RED: Series 012 Ch00 Z00-81
MNs on both ipsi and contra side of brainstem
Overexposed. Splatter visible

Revision as of 13:50, 24 September 2013

NF001 - embryo sb - Stage 20-21 - 2006.08.02

Experiment

Objective Try to differentiate between rostral and caudal components of cochleovestibular ganglion

Procedure

Both dyes inkected on LEFT.

  • DiI crystals (anterior VIII)
  • DiO crystals (posterior VIII)

Notes: Some splatter. Rhombomere boundaries visible and clear

Files

  • nf001 series 012 ch00 z00-81 - Overexposed. Splatter visible

Results

anterior VIII MNs on both ipsi and contra side of brainstem

posterior VIII No labelling

MNs on both ipsi and contra side of brainstem