Kubke Lab:Research/CND/Records/NF002: Difference between revisions

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'''NF002 - embryo sb - Stage 23 (25?) 21 2/8/06'''  
'''NF002 - embryo sb - Stage 23 (25?) 2006.08.02'''
__TOC__
==Experiment==
'''Objective'''
 
'''Procedure'''
 
* DiI crystal VII, RIGHT side. Remove cochlear ganglion, apply DiI crystal on facial, distal to ganglion.
* DiO crystal VIII, LEFT side. Apply to both branches in the posterior bifurcation of the cochleovestibular ganglion
 
'''Notes'''


Rhombomere boundaries almost visible only rostral to VIII
Rhombomere boundaries almost visible only rostral to VIII
Posterior brainch of Cochlear ganglion has two visible branches.


'''Left side:'''''Italic text''
Posterior branch of Cochlear ganglion has two visible branches.  
Remove cochlear ganglion, apply DiI crystal on facial, distal to ganglion.  
 
'''Right side:'''''Italic text''
==Files==
Apply DiO crystal to both branches in the posterior bifurcation of the ganglion
* RED: Series 006 Ch00 Z000-039


'''Results:'''
==Results==
No uptake of DiO
 
* No uptake of DiO


'''Facial labelling'''
'''Facial labelling'''


RED: Series 006 Ch00 Z000-039
MN labelled unilaterally. There is also a thin lateral fascile of short afferent axons on the ipsilateral side.
Stack does not cover full thickness of hindbrain - I am missing the bundle of axons that are going to the motoneurons in the caudal segment.  
Stack does not cover full thickness of hindbrain - I am missing the bundle of axons that are going to the motoneurons in the caudal segment.  


The caudal group of motoneurons seem to have extensions on or crossing the floor plate.  
* '''''Afferents'''''
There is also a thin lateral fascicle of short afferent axons on the ipsilateral side. Rostral sensory fascicle seems separated into 2 discrete bundles. Caudally it seems to be the case too, but they appear to come back together at a more caudal level (not so for rostral bundles).


Rostral sensory fascile seems eparated into 2 discrete bundles. Cuadally it seems to be the case too, but they appear to come back together at a more caudal level (not so for rostral bundles).
*'''''Efferents'''''
MN labelled unilaterally. The caudal group of motoneurons seem to have extensions on or crossing the floor plate.

Latest revision as of 15:50, 7 October 2013

NF002 - embryo sb - Stage 23 (25?) 2006.08.02

Experiment

Objective

Procedure

  • DiI crystal VII, RIGHT side. Remove cochlear ganglion, apply DiI crystal on facial, distal to ganglion.
  • DiO crystal VIII, LEFT side. Apply to both branches in the posterior bifurcation of the cochleovestibular ganglion

Notes

Rhombomere boundaries almost visible only rostral to VIII

Posterior branch of Cochlear ganglion has two visible branches.

Files

  • RED: Series 006 Ch00 Z000-039

Results

  • No uptake of DiO

Facial labelling

Stack does not cover full thickness of hindbrain - I am missing the bundle of axons that are going to the motoneurons in the caudal segment.

  • Afferents

There is also a thin lateral fascicle of short afferent axons on the ipsilateral side. Rostral sensory fascicle seems separated into 2 discrete bundles. Caudally it seems to be the case too, but they appear to come back together at a more caudal level (not so for rostral bundles).

  • Efferents

MN labelled unilaterally. The caudal group of motoneurons seem to have extensions on or crossing the floor plate.

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