Kubke Lab:Research/CND/Records/NF002: Difference between revisions
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'''''Right side:''''' | '''''Right side:''''' | ||
Apply DiO crystal to both branches in the posterior bifurcation of the ganglion | Apply DiO crystal to both branches in the posterior bifurcation of the cochleovestibular ganglion | ||
'''Results:''' | '''Results:''' | ||
Revision as of 13:32, 24 September 2013
NF002 - embryo sb - Stage 23 (25?) 2/8/06
Rhombomere boundaries almost visible only rostral to VIII Posterior branch of Cochlear ganglion has two visible branches.
Left side: Remove cochlear ganglion, apply DiI crystal on facial, distal to ganglion.
Right side: Apply DiO crystal to both branches in the posterior bifurcation of the cochleovestibular ganglion
Results:
No uptake of DiO
Facial labelling
RED: Series 006 Ch00 Z000-039
MN labelled unilaterally. There is also a thin lateral fascicle of short afferent axons on the ipsilateral side. Stack does not cover full thickness of hindbrain - I am missing the bundle of axons that are going to the motoneurons in the caudal segment.
The caudal group of motoneurons seem to have extensions on or crossing the floor plate.
Rostral sensory fascicle seems separated into 2 discrete bundles. Caudally it seems to be the case too, but they appear to come back together at a more caudal level (not so for rostral bundles).
