Kubke Lab:Research/CND/Records/NF002

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NF002 - embryo sb - Stage 23 (25?) 21 2/8/06

Rhombomere boundaries almost visible only rostral to VIII Posterior brainch of Cochlear ganglion has two visible branches.

Left side: Remove cochlear ganglion, apply DiI crystal on facial, distal to ganglion. Right side: Apply DiO crystal to both branches in the posterior bifurcation of the ganglion

Results: No uptake of DiO

Facial labelling: RED: Series 006 Ch00 Z000-039

MN labelled unilaterally. There is also a thin lateral fascile of short afferent axons on the ipsilateral side. Stack does not cover full thickness of hindbrain - I am missing the bundle of axons that are going to the motoneurons in the caudal segment.

The caudal group of motoneurons seem to have extensions on or crossing the floor plate.

Rostral sensory fascile seems eparated into 2 discrete bundles. Cuadally it seems to be the case too, but they appear to come back together at a more caudal level (not so for rostral bundles).

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