Kubke Lab:Research/CND/Records/NF007

NF007 - species - Stage 26 - 2006.08.24

Experiment

Objective Try to get more detailed view of what are the auditory components.

Procedure

• Right: DiI crystal on cochlear branch of caudal branch of cochleovestibular ganglion.
• Left: DiO crystal on cochlear branch of caudal branch of cochleovestibular ganglion.

Notes

• Can still see some segmental boundaries.
• Dissection took forever but it looks quite good.
• Beautiful dye placement of cochlear branch of posterior branch.

Files

• nf007_Series_14 Ch00 Z00-51 (RED)
• nf007_Series_16 Ch00 Z00-51 (GREEN)

Results

DiO did not seem to go in well. There is nice labelling on both sides. Very symmetrical labelling. Looks quite clean and beautiful, except that there is a bit of spill caudal to the VIII n. on the right side which showed up in the confocal.

63x objective not working.

Low mag images only, not a lot of detail.

RIGHT cochlear/lagenar branch of caudal cochleovestibular

Some spill caudal to the nerve entry.

• Afferents

Can't analyse, since I get some labelling from the DiI spill

• Efferents

Very few labelled despite strong labelling at the nerve entry

• Other

The nerve makes a well defined loop coming into the brainstem.

LEFT cochlear/lagenar branch of caudal cochleovestibular

• Afferents

Seem to form a single well defined bundle, one running caudal and one rostral. They obviously extend beyond the segments where the efferents are found.

• Efferents

There is clear label on efferents, especially in r4, although some r5 can be seem too, but there seem to be less of those.

• Other

The nerve makes a well defined loop coming into the brainstem.

Summary

Questions

Future