Kubke Lab:Research/CND/Records/NF007: Difference between revisions
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DiO did not seem to go in well. There is nice labelling on both sides. Very symmetrical labelling. Looks quite clean and beautiful, except that there is a bit of spill caudal to the VIII n. on the right side which showed up in the confocal. | DiO did not seem to go in well. There is nice labelling on both sides. Very symmetrical labelling. Looks quite clean and beautiful, except that there is a bit of spill caudal to the VIII n. on the right side which showed up in the confocal. | ||
=== | 63x objective not working. | ||
Low mag images only, not a lot of detail. | |||
===RIGHT cochlear/lagenar branch of caudal cochleovestibular=== | |||
Some spill caudal to the nerve entry. | |||
* '''''Afferents''''' | * '''''Afferents''''' | ||
Can't analyse, since I get some labelling from the DiI spill | |||
* '''''Efferents''''' | * '''''Efferents''''' | ||
Very few labelled despite strong labelling at the nerve entry | |||
* '''''Other''''' | * '''''Other''''' | ||
=== | The nerve makes a well defined loop coming into the brainstem. | ||
===LEFT cochlear/lagenar branch of caudal cochleovestibular=== | |||
* '''''Afferents''''' | * '''''Afferents''''' | ||
Seem to form a single well defined bundle, one running caudal and one rostral. They obviously extend beyond the segments where the efferents are found. | |||
* '''''Efferents''''' | * '''''Efferents''''' | ||
There is clear label on efferents, especially in r4, although some r5 can be seem too, but there seem to be less of those. | |||
* '''''Other''''' | * '''''Other''''' | ||
The nerve makes a well defined loop coming into the brainstem. | |||
==Summary== | ==Summary== | ||
Revision as of 16:50, 7 October 2013
NF007 - species - Stage 26 - 206.08.24
Experiment
Objective Try to get more detailed view of what are the auditory components.
Procedure
- Right: DiI crystal on cochlear branch of caudal branch of cochleovestibular ganglion.
- Left: DiO crystal on cochlear branch of caudal branch of cochleovestibular ganglion.
Notes
- Can still see some segmental boundaries.
- Dissection took forever but it looks quite good.
- Beautiful dye placement of cochlear branch of posterior branch.
Files
- nf007_Series_14 Ch00 Z00-51 (RED) Odd angle?
- nf007_Series_16 Ch00 Z00-51
Results
DiO did not seem to go in well. There is nice labelling on both sides. Very symmetrical labelling. Looks quite clean and beautiful, except that there is a bit of spill caudal to the VIII n. on the right side which showed up in the confocal.
63x objective not working.
Low mag images only, not a lot of detail.
RIGHT cochlear/lagenar branch of caudal cochleovestibular
Some spill caudal to the nerve entry.
- Afferents
Can't analyse, since I get some labelling from the DiI spill
- Efferents
Very few labelled despite strong labelling at the nerve entry
- Other
The nerve makes a well defined loop coming into the brainstem.
LEFT cochlear/lagenar branch of caudal cochleovestibular
- Afferents
Seem to form a single well defined bundle, one running caudal and one rostral. They obviously extend beyond the segments where the efferents are found.
- Efferents
There is clear label on efferents, especially in r4, although some r5 can be seem too, but there seem to be less of those.
- Other
The nerve makes a well defined loop coming into the brainstem.
Summary
Questions
Future
