Kubke Lab:Research/CND/Records/NF007

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==Files==
==Files==
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*nf007_Series_14 Ch00 Z00-51 (RED) Odd angle?
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*nf007_Series_14 Ch00 Z00-51 (RED)
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*nf007_Series_16 Ch00 Z00-51
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*nf007_Series_16 Ch00 Z00-51 (GREEN)
==Results==
==Results==

Revision as of 19:50, 7 October 2013

Contents


NF007 - species - Stage 26 - 206.08.24


Experiment

Objective Try to get more detailed view of what are the auditory components.

Procedure

  • Right: DiI crystal on cochlear branch of caudal branch of cochleovestibular ganglion.
  • Left: DiO crystal on cochlear branch of caudal branch of cochleovestibular ganglion.

Notes

  • Can still see some segmental boundaries.
  • Dissection took forever but it looks quite good.
  • Beautiful dye placement of cochlear branch of posterior branch.

Files

  • nf007_Series_14 Ch00 Z00-51 (RED)
  • nf007_Series_16 Ch00 Z00-51 (GREEN)

Results

DiO did not seem to go in well. There is nice labelling on both sides. Very symmetrical labelling. Looks quite clean and beautiful, except that there is a bit of spill caudal to the VIII n. on the right side which showed up in the confocal.

63x objective not working.

Low mag images only, not a lot of detail.

RIGHT cochlear/lagenar branch of caudal cochleovestibular

Some spill caudal to the nerve entry.

  • Afferents

Can't analyse, since I get some labelling from the DiI spill

  • Efferents

Very few labelled despite strong labelling at the nerve entry

  • Other

The nerve makes a well defined loop coming into the brainstem.

LEFT cochlear/lagenar branch of caudal cochleovestibular

  • Afferents

Seem to form a single well defined bundle, one running caudal and one rostral. They obviously extend beyond the segments where the efferents are found.

  • Efferents

There is clear label on efferents, especially in r4, although some r5 can be seem too, but there seem to be less of those.

  • Other

The nerve makes a well defined loop coming into the brainstem.

Summary

Questions

Future

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