Kubke Lab:Research/CND/Records2010-2011Summer

Experimental records for the Cranial Nerve Development Project

This page lists and links to the detailed experimental records used in this project.

Embryo name	Stage	Date	Procedure	Nerve	Comments
MH001	ST28	25/11/2010	Cryostat Tutorial	N/A	Stage confirmed by Fabiana
MH002	ST28	3/12/2010	Assessing cryostat parameters for ST28	N/A	H&E stained slides.Poor quality
MH003	ST20-22	13/12/2010	Fabiana's cryostat session	N/A	N/A
MH004	ST28-29	16/12/2010	Assessing cryostat parameters for ST28	N/A	Will be updating feedback from results
MH005	ST28-29	14/1/2010	Cutting session	N/A	Unstained sections. Superfrost slides
MH006	ST28	19/1/2011	Cutting session	N/A	H&E stained sections , poor tissue quality.
MH007	ST25				Only staged has not been cut yet
MH008	ST19	26/01/2011	Serial Nissl sections		Complete Nissl stained serial sections
RC001	ST22	23/11/10	Histology Training with Satya		Evaluating a protocol to follow to prepare stained serial sections.
RC002	ST23	2/12/2010	Histology Training with Satya		Evaluating a protocol to follow to prepare stained serial sections.
RC003	ST21	10/01/2011	Staging Exercise for Reuben, sectioned by Fabiana, stained by Reuben		Following staining decided to not use H&E staining.
RC004	ST25	17/12/2010	Histology Training with Satya		Some tissue sections good, derived sectioning protocol from these results.
RC005	ST19	10/01/2011			.
RC006	ST20	Staged:10/01/2011, Cutting: 13/01/2011	Sectioned then Nissl stained.		Un-subbed slides were used and the tissues were moving during coverslipping.
RC007	ST19	10/01/2011	Staging Exercise		Staging unconfirmed.
RC008	ST24	10/01/2011	Staging Exercise		Staging unconfirmed.
RC009	ST24		Sectioned then Nissl stained		Staging unconfirmed, hindbrain not fully cut.Tissue adhering to slide however.
RC010	ST20	10/01/2011	Staging Exercise		Staging unconfirmed.
RC011	ST26	10/01/2011	Staging Exercise		Staging unconfirmed.
RC012	ST29	10/01/2011	Staging Exercise		Staging unconfirmed.
RC013	ST30	10/01/2011	Staging Exercise		Staging unconfirmed.
RC014	ST31	10/01/2011	Staging Exercise		Staging unconfirmed.
RC015	ST20+	10/01/2011	Staging Exercise		Staging confirmed, embryo sectioned by Malisha, Nissl stained by Reuben, deemed 'good' but not great quality tissue sections by Fabiana.