Kubke Lab:Research/CND/Records2010-2011Summer

Experimental records for the Cranial Nerve Development Project- 2010-2011 Summer research

This page lists and links to the detailed experimental records for Summer Research 2010-2011

Embryo name	Stage	Date	Procedure	Nerve	Comments
MH001	ST28	25/11/2010	Cryostat Tutorial	N/A	Stage confirmed by Fabiana
MH002	ST28	3/12/2010	Assessing cryostat parameters for ST28	N/A	H&E stained slides.Poor quality
MH003	ST20-22	13/12/2010	Fabiana's cryostat session	N/A	N/A
MH004	ST28-29	16/12/2010	Fabiana's cryostat session	N/A	feedback from results found here
MH005	ST28-29	14/1/2010	Cutting session	N/A	Unstained sections. Superfrost slides
MH006	ST28	19/1/2011	Cutting session	N/A	poor tissue quality.
MH007	ST25				Only staged has not been cut yet
MH008	ST19	26/01/2011	Serial Nissl sections		Complete Nissl stained serial sections
RC001	ST22	23/11/10	Histology Training with Satya		Evaluating a protocol to follow to prepare stained serial sections.
RC002	ST23	2/12/2010	Histology Training with Satya		Evaluating a protocol to follow to prepare stained serial sections.
RC003	ST21	10/01/2011	Staging Exercise for Reuben, sectioned by Fabiana, stained by Reuben		Following staining decided to not use H&E staining.
RC004	ST25	17/12/2010	Histology Training with Satya		Some tissue sections good, derived sectioning protocol from these results.
RC005	ST19	10/01/2011			.
RC006	ST20	Staged:10/01/2011, Cutting: 13/01/2011	Sectioned then Nissl stained.		Un-subbed slides were used and the tissues were moving during coverslipping.
RC007	ST19	10/01/2011	Staging Exercise		Staging unconfirmed.
RC008	ST24	10/01/2011	Staging Exercise		Staging unconfirmed.
RC009	ST24		Sectioned then Nissl stained		Staging unconfirmed, hindbrain not fully cut.Tissue adhering to slide however.
RC010	ST20	10/01/2011	Staging Exercise		Staging unconfirmed.
RC011	ST26	10/01/2011	Staging Exercise		Staging unconfirmed.
RC012	ST29	10/01/2011	Staging Exercise		Staging unconfirmed.
RC013	ST30	10/01/2011	Staging Exercise		Staging unconfirmed.
RC014	ST31	10/01/2011	Staging Exercise		Staging unconfirmed.
RC015	ST20+	10/01/2011	Staging Exercise		Staging confirmed, embryo sectioned by Malisha, Nissl stained by Reuben, deemed 'good' but not great quality tissue sections by Fabiana.