Kubke Lab:Research/CND/Records2010-2011Summer/RC005: Difference between revisions
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<br>'''Embryo Name''': RC005 | <br>'''Embryo Name''': RC005 | ||
<br>'''Embryo stage''': ST20 | <br>'''Embryo stage''': ST20 | ||
''Fabiana staged this embryo on the 25/1 and confirmed it was a ST19.'' --[[User:Malisha Hettiarachchi|Malisha Hettiarachchi]] 15:22, 27 January 2011 (EST) | ''Fabiana staged this embryo on the [[Kubke_Lab:/Notebook/Cranial_nerve_development/2011/01/25|25/1]] and confirmed it was a ST19.'' --[[User:Malisha Hettiarachchi|Malisha Hettiarachchi]] 15:22, 27 January 2011 (EST) | ||
<br>'''Staging description''': Staged by Reuben according to the [http://homepage.univie.ac.at/brian.metscher/Hamburger51_ChickStages.pdf Hamburger and Hamilton (1951) staging system.] | <br>'''Staging description''': Staged by Reuben according to the [http://homepage.univie.ac.at/brian.metscher/Hamburger51_ChickStages.pdf Hamburger and Hamilton (1951) staging system.] | ||
*Wing and leg buds barely formed. | *Wing and leg buds barely formed. | ||
Revision as of 14:17, 27 January 2011
| Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC005
Embryo stage: ST20
Fabiana staged this embryo on the 25/1 and confirmed it was a ST19. --Malisha Hettiarachchi 15:22, 27 January 2011 (EST)
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system.
- Wing and leg buds barely formed.
- The leg is slightly longer than the wing.
- Allantois has
vesicularizedand is roughly the same size as the midbrain (indicative of ST20).
- (Note: what do you mean by vesicularised? --MF Kubke 00:39, 27 January 2011 (EST))
(see Notebook entry 10th January 2011)
Fixation:
Cryoprotection: Was stored in PFA, staged in .9% saline and then replenished with PFA.
Material label and storage:Stored in vial filled with PFA labeled RC005 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST20.
Images
Summary
This embryo may be used in further experiments as approximately a ST20 however confirmation is needed.
