Kubke Lab:Research/CND/Records2010-2011Summer/RC007: Difference between revisions
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*30-40 somites {{Note|Did you count them? --[[User:M Fabiana Kubke|MF Kubke]] 02:30, 27 January 2011 (EST)}} | *30-40 somites {{Note|Did you count them? --[[User:M Fabiana Kubke|MF Kubke]] 02:30, 27 January 2011 (EST)}} | ||
*Tail bud is curved | *Tail bud is curved | ||
<br>'''Fixation''': | |||
<br>'''Cryoprotection''': <del>Was stored in PFA, staged in 0.9% saline and then replenished with PFA. </del>{{Note|this is not cryoprotection --[[User:M Fabiana Kubke|MF Kubke]] 02:29, 27 January 2011 (EST)}} | <br>'''Cryoprotection''': <del>Was stored in PFA, staged in 0.9% saline and then replenished with PFA. </del>{{Note|this is not cryoprotection --[[User:M Fabiana Kubke|MF Kubke]] 02:29, 27 January 2011 (EST)}} | ||
<br>'''Material label and storage''': Stored in vial filled with PFA labeled RC-007 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope. | <br>'''Material label and storage''': Stored in vial filled with PFA labeled RC-007 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope. |
Revision as of 01:47, 27 January 2011
Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC007
Embryo stage: ST19
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. Stage not confirmed. (Notebook entry 10th January 2011)
- Small allantois (not nearly as big as the midbrain, <ST20)
- (Note: Since I conducted this staging exercise Fabiana alerted me to the problematic approach of including allantois morphology in my staging decision unless I am using fresh tissue.)
- Trunk flexure has disappeared (absent) (>ST18)
- 30-40 somites
- (Note: Did you count them? --MF Kubke 02:30, 27 January 2011 (EST))
- Tail bud is curved
Fixation:
Cryoprotection: Was stored in PFA, staged in 0.9% saline and then replenished with PFA.
- (Note: this is not cryoprotection --MF Kubke 02:29, 27 January 2011 (EST))
Material label and storage: Stored in vial filled with PFA labeled RC-007 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST19
Images
Summary
This embryo may be used in further experiments as approximately a ST19 embryo however confirmation is needed.