Kubke Lab:Research/CND/Records2010-2011Summer/RC007

Cranial Nerve Development

Experiment

Embryo details

Species: Gallus gallus domesticus
Embryo Name: RC007
Embryo stage: ST19
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. Stage not confirmed. (Notebook entry 10th January 2011)

• Small allantois (not nearly as big as the midbrain, <ST20)

(Note: Since I conducted this staging exercise Fabiana alerted me to the problematic approach of including allantois morphology in my staging decision unless I am using fresh tissue.)

• Trunk flexure has disappeared (absent) (>ST18)
• 30-40 somites

(Note: Did you count them? --MF Kubke 02:30, 27 January 2011 (EST))

(Note: Thought I did, are you suggesting it's unlikely I was counting somites?)

• Tail bud is curved

Fixation: PFA
Cryoprotection: None.
Material label and storage: Stored in vial filled with PFA labeled RC-007 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.

Experiment details

Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:

Results

Embryo is approximately ST19

Summary

This embryo may be used in further experiments as approximately a ST19 embryo however confirmation is needed.