Kubke Lab:Research/CND/Records2010-2011Summer/RC008: Difference between revisions
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=Experiment details= | =Experiment details= | ||
Date: 10th January 2011 see [[Kubke Lab:/Notebook/Cranial nerve development/2011/01/10|Notebook entry]] | '''Date''': 10th January 2011 see [[Kubke Lab:/Notebook/Cranial nerve development/2011/01/10|Notebook entry]] | ||
<br>Objective:To use as a practice embryo for staging. | <br>'''Objective''': To use as a practice embryo for staging. | ||
<br>Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage. | <br>'''Procedure''': Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage. | ||
<br>Comments: | <br>'''Comments''': | ||
=Results= | =Results= | ||
Revision as of 00:27, 27 January 2011
| Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC008
Embryo stage: ST24
Staging description:Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. Staging has not been confirmed.
- Eye is pigmented (>ST21).
- Leg bud and wing bud are roughly the same size.
- Limb buds are distinctively longer than they are wider.
- Dorsal contour of trunk is curved.
- 2nd Visceral arch distinctly protrudes across the ventral surface of embryo.
Cryoprotection: Was stored in PFA, staged in .9% saline and then replenished with PFA.
Material label and storage: Stored in vial filled with PFA labeled RC-008 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Date: 10th January 2011 see Notebook entry
Objective: To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST24
Images
Summary
This embryo may be used in further experiments as approximately a ST24 embryo however confirmation is needed.
