Kubke Lab:Research/CND/Records2010-2011Summer/RC014: Difference between revisions
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*Legs match ST30-ST31. | *Legs match ST30-ST31. | ||
*Lines within the eye are present pigmentation matches 31 more closely. | *Lines within the eye are present pigmentation matches 31 more closely. | ||
<br>'''Fixation''': | |||
<br>'''Cryoprotection''': Was stored in PFA, staged in .9% saline and then replenished with PFA. | <br>'''Cryoprotection''': Was stored in PFA, staged in .9% saline and then replenished with PFA. | ||
<br>'''Material label and storage''': Stored in vial filled with PFA labeled ST31 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope. | <br>'''Material label and storage''': Stored in vial filled with PFA labeled ST31 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope. | ||
Revision as of 02:01, 27 January 2011
| Cranial Nerve Development | Experiment |
Embryo details
Date: 10th January 2011 see Notebook entry
Species: Gallus gallus domesticus
Embryo Name: RC014
Embryo stage: ST31
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. Staging has not been confirmed.
- Beak morphology matches ST30-ST31.
- Legs match ST30-ST31.
- Lines within the eye are present pigmentation matches 31 more closely.
Fixation:
Cryoprotection: Was stored in PFA, staged in .9% saline and then replenished with PFA.
Material label and storage: Stored in vial filled with PFA labeled ST31 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective: To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST31.
Images
Summary
This embryo may be used in further experiments as approximately a ST31 embryo however confirmation is needed.
