Kubke Lab:Research/CND/Summary VIII

stage 26

case nf005

(GREEN) Nerve entry well differentiated from VII nerve, entering in a more dorsolateral position with respect to VII.

Both rostral and caudal sensory bundles seem to be split. The rostral bundle seems to be split into a longer thinner dorsolateral bundle and a seemingly less fasciculated medial bundle. The caudal bundle seems to be split into two and perhaps 3 bundles. Both bundles rostral and caudal show perpendicular projections of axons coursing towards the midline.

Efferent neurons are found in both r4-r5 and appear to occupy a more ventral position than the VII efferents. Labelling appears to be more intense in r4, where there seem to be two groups - a more dorsal one in a more lateral position and a more ventral one with cells all the way to the floor plate.

High magnification of the floor plate also shows extensions crossing the floor plate, as well as some fibres that seem to be coursing rostrocaudally at the edge of the floor plate.

There are also clearly labelled cell bodies in the dorsolateral edge of the wholemount, most likely in the alar plate. These lateral cell bodies can be found in r4 and r5 but they also seem to be present in r3.