LB
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(Difference between revisions)
| Line 11: | Line 11: | ||
#Autoclave (liquid cycle) | #Autoclave (liquid cycle) | ||
#* 250°'''F''', 22psi, 30 minutes | #* 250°'''F''', 22psi, 30 minutes | ||
| + | ==Source== | ||
| + | J. Sambrook, D.W. Russell, ''Molecular Cloning: A Laboratory Manual'' (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) | ||
Revision as of 16:01, 3 June 2005
Contents |
Summary
L-Broth or LB Medium. Standard growth medium for a variety of bacteria and conditions.
Ingredients
- 10 g Bacto-tryptone
- 5 g yeast extract
- 10 g NaCl
- distilled water
Protocol
- Mix dry ingredients and add distilled water up to 1 Liter
- Pour into 2 L flask (or greater)
- Autoclave (liquid cycle)
- 250°F, 22psi, 30 minutes
Source
J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001)
