LB

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 5: Line 5:
#5 g yeast extract
#5 g yeast extract
#10 g NaCl
#10 g NaCl
-
#distilled water
 
==Protocol==
==Protocol==
#Mix dry ingredients and add distilled water up to 1 Liter
#Mix dry ingredients and add distilled water up to 1 Liter
Line 12: Line 11:
#* 250°'''F''', 22psi, 30 minutes
#* 250°'''F''', 22psi, 30 minutes
==Source==
==Source==
-
J. Sambrook, D.W. Russell, ''Molecular Cloning: A Laboratory Manual'' (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001)
+
Adapted From:
 +
 
 +
J. Sambrook, D.W. Russell, ''Molecular Cloning: A Laboratory Manual'' (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) pg. A2.2

Revision as of 15:05, 3 June 2005

Contents

Summary

L-Broth or LB Medium. Standard growth medium for a variety of bacteria and conditions.

Ingredients

  1. 10 g Bacto-tryptone
  2. 5 g yeast extract
  3. 10 g NaCl

Protocol

  1. Mix dry ingredients and add distilled water up to 1 Liter
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes

Source

Adapted From:

J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, ed. 3, 2001) pg. A2.2

Personal tools