LabName:Supplementary

From OpenWetWare
Jump to navigationJump to search

Home        Team        Project        Experiment        Supplementary        Sponsors       


Details of Spider Silk

Cadnano designs

  • ▲ Cadnano design for Brick

    ▲ Cadnano design for Brick

  • ▲ Cadnano design for Connector

    ▲ Cadnano design for Connector


Sequence

scaffold

  • Scaffold of 4539NT

GATCGTTACGCTAACTATGAGGGCTGTCTGTGGAATGCTACAGGCGTTGTAGTTTGTACTGGTGACGAAACTCAGTGTTACGGTACATGGGTTCCTATT GGGCTTGCTATCCCTGAAAATGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGTTCTGAGGGTGGCGGTACTAAACCTCCTGAGTACGGT GATACACCTATTCCGGGCTATACTTATATCAACCCTCTCGACGGCACTTATCCGCCTGGTACTGAGCAAAACCCCGCTAATCCTAATCCTTCTCTTGAG GAGTCTCAGCCTCTTAATACTTTCATGTTTCAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTGTTTATACGGGCACTGTTACTCAAGGCACT GACCCCGTTAAAACTTATTACCAGTACACTCCTGTATCATCAAAAGCCATGTATGACGCTTACTGGAACGGTAAATTCAGAGACTGCGCTTTCCATTCT GGCTTTAATGAGGATTTATTTGTTTGTGAATATCAAGGCCAATCGTCTGACCTGCCTCAACCTCCTGTCAATGCTGGCGGCGGCTCTGGTGGTGGTTCT GGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGGTTCCGGTGATTTT GATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAATGCCGATGAAAACGCGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTC GCTACTGATTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGGTGCTACTGGTGATTTTGCTGGCTCTAATTCC CAAATGGCTCAAGTCGGTGACGGTGATAATTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATGTCGCCCTTTT GTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAATAAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTT ATGTATGTATTTTCTACGTTTGCTAACATACTGCGTAATAAGGAGTCTTAATCATGCCAGTTCTTTTGGGTATTCCGTTATTATTGCGTTTCCTCGGTT TCCTTCTGGTAACTTTGTTCGGCTATCTGCTTACTTTTCTTAAAAAGGGCTTCGGTAAGATAGCTATTGCTATTTCATTGTTTCTTGCTCTTATTATTG GGCTTAACTCAATTCTTGTGGGTTATCTCTCTGATATTAGCGCTCAATTACCCTCTGACTTTGTTCAGGGTGTTCAGTTAATTCTCCCGTCTAATGCGC TTCCCTGTTTTTATGTTATTCTCTCTGTAAAGGCTGCTATTTTCATTTTTGACGTTAAACAAAAAATCGTTTCTTATTTGGATTGGGATAAATAATATG GCTGTTTATTTTGTAACTGGCAAATTAGGCTCTGGAAAGACGCTCGTTAGCGTTGGTAAGATTCAGGATAAAATTGTAGCTGGGTGCAAAATAGCAACT AATCTTGATTTAAGGCTTCAAAACCTCCCGCAAGTCGGGAGGTTCGCTAAAACGCCTCGCGTTCTTAGAATACCGGATAAGCCTTCTATATCTGATTTG CTTGCTATTGGGCGCGGTAATGATTCCTACGATGAAAATAAAAACGGCTTGCTTGTTCTCGATGAGTGCGGTACTTGGTTTAATACCCGTTCTTGGAAT GATAAGGAAAGACAGCCGATTATTGATTGGTTTCTACATGCTCGTAAATTAGGATGGGATATTATTTTTCTTGTTCAGGACTTATCTATTGTTGATAAA CAGGCGCGTTCTGCATTAGCTGAACATGTTGTTTATTGTCGTCGTCTGGACAGAATTACTTTACCTTTTGTCGGTACTTTATATTCTCTTATTACTGGC TCGAAAATGCCTCTGCCTAAATTACATGTTGGCGTTGTTAAATATGGCGATTCTCAATTAAGCCCTACTGTTGAGCGTTGGCTTTATACTGGTAAGAAT TTGTATAACGCATATGATACTAAACAGGCTTTTTCTAGTAATTATGATTCCGGTGTTTATTCTTATTTAACGCCTTATTTATCACACGGTCGGTATTTC AAACCATTAAATTTAGGTCAGAAGATGAAATTAACTAAAATATATTTGAAAAAGTTTTCTCGCGTTCTTTGTCTTGCGATTGGATTTGCATCAGCATTT ACATATAGTTATATAACCCAACCTAAGCCGGAGGTTAAAAAGGTAGTCTCTCAGACCTATGATTTTGATAAATTCACTATTGACTCTTCTCAGCGTCTT AATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAATAGCGACGATTTACAGAAGCAAGGTTATTCACTCACATATATTGATTTA TGTACTGTTTCCATTAAAAAAGGTAATTCAAATGAAATTGTTAAATGTAATTAATTTTGTTTTCTTGATGTTTGTTTCATCATCTTCTTTTGCTCAGGT AATTGAAATGAATAATTCGCCTCTGCGCGATTTTGTAACTTGGTATTCAAAGCAATCAGGCGAATCCGTTATTGTTTCTCCCGATGTAAAAGGTACTGT TACTGTATATTCATCTGACGTTAAACCTGAAAATCTACGCAATTTCTTTATTTCTGTTTTACGTGCAAATAATTTTGATATGGTAGGTTCTAACCCTTC CATTATTCAGAAGTATAATCCAAACAATCAGGATTATATTGATGAATTGCCATCATCTGATAATCAGGAATATGATGATAATTCCGCTCCTTCTGGTGG TTTCTTTGTTCCGCAAAATGATAATGTTACTCAAACTTTTAAAATTAATAACGTTCGGGCAAAGGATTTAATACGAGTTGTCGAATTGTTTGTAAAGTC TAATACTTCTAAATCCTCAAATGTATTATCTATTGACGGCTCTAATCTATTAGTTGTTAGTGCTCCTAAAGATATTTTAGATAACCTTCCTCAATTCCT TTCAACTGTTGATTTGCCAACTGACCAGATATTGATTGAGGGTTTGATATTTGAGGTTCAGCAAGGTGATGCTTTAGATTTTTCATTTGCTGCTGGCTC TCAGCGTGGCACTGTTGCAGGCGGTGTTAATACTGACCGCCTCACCTCTGTTTTATCTTCTGCTGGTGGTTCGTTCGGTATTTTTAATGGCGATGTTTT AGGGCTATCAGTTCGCGCATTAAAGACTAATAGCCATTCAAAAATATTGTCTGTGCCACGTATTCTTACGCTTTCAGGTCAGAAGGGTTCTATCTCTGT TGGCCAGAATGTCCCTTTTATTACTGGTCGTGTGACTGGTGAATCTGCCAATGTAAATAATCCATTTCAGACGATTGAGCGTCAAAATGTAGGTATTTC CATGAGCGTTTTTCCTGTTGCAATGGCTGGCGGTAATATTGTTCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGTGATGT TATTACTAATCAAAGAAGTATTGCTACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACTCGGTGGCCTCACTGATTATAAAAACACTTCTCAGGA TTCTGGCGTACCGTTCCTGTCTAAAATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTGATTCTAACGAGGAAAGCACGTTATACGTGCTCGTCAA AGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCG CTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTT TACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCA CGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGAAC CACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCC CGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGT TTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTA TGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCGAGCTCGGTACCCGGG

  • Scaffold of 5081NT

AATGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAATATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCT AATGGTCAAACTAAATCTACTCGTTCGCAGAATTGGGAATCAACTGTTATATGGAATGAAACTTCCAGACACCGTACTTTAGTTGCATATTTAAAACAT GTTGAGCTACAGCATTATATTCAGCAATTAAGCTCTAAGCCATCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGAC CTGTTGGAGTTTGCTTCCGGTCTGGTTCGCTTTGAAGCTCGAATTAAAACGCGATATTTGAAGTCTTTCGGGCTTCCTCTTAATCTTTTTGATGCAATC CGCTTTGCTTCTGACTATAATAGTCAGGGTAAAGACCTGATTTTTGATTTATGGTCATTCTCGTTTTCTGAACTGTTTAAAGCATTTGAGGGGGATTCA ATGAATATTTATGACGATTCCGCAGTATTGGACGCTATCCAGTCTAAACATTTTACTATTACCCCCTCTGGCAAAACTTCTTTTGCAAAAGCCTCTCGC TATTTTGGTTTTTATCGTCGTCTGGTAAACGAGGGTTATGATAGTGTTGCTCTTACTATGCCTCGTAATTCCTTTTGGCGTTATGTATCTGCATTAGTT GAATGTGGTATTCCTAAATCTCAACTGATGAATCTTTCTACCTGTAATAATGTTGTTCCGTTAGTTCGTTTTATTAACGTAGATTTTTCTTCCCAACGT CCTGACTGGTATAATGAGCCAGTTCTTAAAATCGCATAAGGTAATTCACAATGATTAAAGTTGAAATTAAACCATCTCAAGCCCAATTTACTACTCGTT CTGGTGTTTCTCGTCAGGGCAAGCCTTATTCACTGAATGAGCAGCTTTGTTACGTTGATTTGGGTAATGAATATCCGGTTCTTGTCAAGATTACTCTTG ATGAAGGTCAGCCAGCCTATGCGCCTGGTCTGTACACCGTTCATCTGTCCTCTTTCAAAGTTGGTCAGTTCGGTTCCCTTATGATTGACCGTCTGCGCC TCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAATTTATCAGGCGATGATACAAATCTCCGTTGTACTTTGTTTCGCGCTTGGTATA ATCGCTGGGGGTCAAAGATGAGTGTTTTAGTGTATTCTTTTGCCTCTTTCGTTTTAGGTTGGTGCCTTCGTAGTGGCATTACGTATTTTACCCGTTTAA TGGAAACTTCCTCATGAAAAAGTCTTTAGTCCTCAAAGCCTCTGTAGCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGCTGAGGGTGACGATCC CGCAAAAGCGGCCTTTAACTCCCTGCAAGCCTCAGCGACCGAATATATCGGTTATGCGTGGGCGATGGTTGTTGTCATTGTCGGCGCAACTATCGGTAT CAAGCTGTTTAAGAAATTCACCTCGAAAGCAAGCTGATAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTGGAGATTTTCAACGTGAAAA AATTATTATTCGCAATTCCTTTAGTTGTTCCTTTCTATTCTCACTCCGCTGAAACTGTTGAAAGTTGTTTAGCAAAATCCCATACAGAAAATTCATTTA CTAACGTCTGGAAAGACGACAAAACTTTAGATCGTTACGCTAACTATGAGGGCTGTCTGTGGAATGCTACAGGCGTTGTAGTTTGTACTGGTGACGAAA CTCAGTGTTACGGTACATGGGTTCCTATTGGGCTTGCTATCCCTGAAAATGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGTTCTGAGG GTGGCGGTACTAAACCTCCTGAGTACGGTGATACACCTATTCCGGGCTATACTTATATCAACCCTCTCGACGGCACTTATCCGCCTGGTACTGAGCAAA ACCCCGCTAATCCTAATCCTTCTCTTGAGGAGTCTCAGCCTCTTAATACTTTCATGTTTCAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTG TTTATACGGGCACTGTTACTCAAGGCACTGACCCCGTTAAAACTTATTACCAGTACACTCCTGTATCATCAAAAGCCATGTATGACGCTTACTGGAACG GTAAATTCAGAGACTGCGCTTTCCATTCTGGCTTTAATGAGGATTTATTTGTTTGTGAATATCAAGGCCAATCGTCTGACCTGCCTCAACCTCCTGTCA ATGCTGGCGGCGGCTCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTT CCGGTGGTGGCTCTGGTTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAATGCCGATGAAAACGCGCTACAGT CTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGATTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGGTG CTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAATTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCC TCCCTCAATCGGTTGAATGTCGCCCTTTTGTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAATAAACTTATTCCGTGGTGTCT TTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATTTTCTACGTTTGCTAACATACTGCGTAATAAGGAGTCTTAATCATGCCAGTTCTTTTGGG TATTCCGTTATTATTGCGTTTCCTCGGTTTCCTTCTGGTAACTTTGTTCGGCTATCTGCTTACTTTTCTTAAAAAGGGCTTCGGTAAGATAGCTATTGC TATTTCATTGTTTCTTGCTCTTATTATTGGGCTTAACTCAATTCTTGTGGGTTATCTCTCTGATATTAGCGCTCAATTACCCTCTGACTTTGTTCAGGG TGTTCAGTTAATTCTCCCGTCTAATGCGCTTCCCTGTTTTTATGTTATTCTCTCTGTAAAGGCTGCTATTTTCATTTTTGACGTTAAACAAAAAATCGT TTCTTATTTGGATTGGGATAAATAATATGGCTGTTTATTTTGTAACTGGCAAATTAGGCTCTGGAAAGACGCTCGTTAGCGTTGGTAAGATTCAGGATA AAATTGTAGCTGGGTGCAAAATAGCAACTAATCTTGATTTAAGGCTTCAAAACCTCCCGCAAGTCGGGAGGTTCGCTAAAACGCCTCGCGTTCTTAGAA TACCGGATAAGCCTTCTATATCTGATTTGCTTGCTATTGGGCGCGGTAATGATTCCTACGATGAAAATAAAAACGGCTTGCTTGTTCTCGATGAGTGCG GTACTTGGTTTAATACCCGTTCTTGGAATGATAAGGAAAGACAGCCGATTATTGATTGGTTTCTACATGCTCGTAAATTAGGATGGGATATTATTTTTC TTGTTCAGGACTTATCTATTGTTGATAAACAGGCGCGTTCTGCATTAGCTGAACATGTTGTTTATTGTCGTCGTCTGGACAGAATTACTTTACCTTTTG TCGGTACTTTATATTCTCTTATTACTGGCTCGAAAATGCCTCTGCCTAAATTACATGTTGGCGTTGTTAAATATGGCGATTCTCAATTAAGCCCTACTG TTGAGCGTTGGCTTTATACTGGTAAGAATTTGTATAACGCATATGATACTAAACAGGCTTTTTCTAGTAATTATGATTCCGGTGTTTATTCTTATTTAA CGCCTTATTTATCACACGGTCGGTATTTCAAACCATTAAATTTAGGTCAGAAGATGAAATTAACTAAAATATATTTGAAAAAGTTTTCTCGCGTTCTTT GTCTTGCGATTGGATTTGCATCAGCATTTACATATAGTTATATAACCCAACCTAAGCCGGAGGTTAAAAAGGTAGTCTCTCAGACCTATGATTTTGATA AATTCACTATTGACTCTTCTCAGCGTCTTAATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAATAGCGACGATTTACAGAAGC AAGGTTATTCACTCACATATATTGATTTATGTACTGTTTCCATTAAAAAAGGTAATTCAAATGAAATTGTTAAATGTAATTAATTTTGTTTTCTTGATG TTTGTTTCATCATCTTCTTTTGCTCAGGTAATTGAAATGAATAATTCGCCTCTGCGCGATTTTGTAACTTGGTATTCAAAGCAATCAGGCGAATCCGTT ATTGTTTCTCCCGATGTAAAAGGTACTGTTACTGTATATTCATCTGACGTTAAACCTGAAAATCTACGCAATTTCTTTATTTCTGTTTTACGTGCAAAT AATTTTGATATGGTAGGTTCTAACCCTTCCATTATTCAGAAGTATAATCCAAACAATCAGGATTATATTGATGAATTGCCATCATCTGATAATCAGGAA TATGATGATAATTCCGCTCCTTCTGGTGGTTTCTTTGTTCCGCAAAATGATAATGTTACTCAAACTTTTAAAATTAATAACGTTCGGGCAAAGGATTTA ATACGAGTTGTCGAATTGTTTGTAAAGTCTAATACTTCTAAATCCTCAAATGTATTATCTATTGACGGCTCTAATCTATTAGTTGTTAGTGCTCCTAAA GATATTTTAGATAACCTTCCTCAATTCCTTTCAACTGTTGATTTGCCAACTGACCAGATATTGATTGAGGGTTTGATATTTGAGGTTCAGCAAGGTGAT GCTTTAGATTTTTCATTTGCTGCTGGCTCTCAGCGTGGCACTGTTGCAGGCGGTGTTAATACTGACCGCCTCACCTCTGTTTTATCTTCTGCTGGTGGT TCGTTCGGTATTTTTAATGGCGATGTTTTAGGGCTATCAGTTCGCGCATTAAAGACTAATAGCCATTCAAAAATATTGTCTGTGCCACGTATTCTTACG
CTTTCAGGTCAGAAGGGTTCTATCTCTGTTGG

Staple

Staple of 4539NT
Start End Sequence
1[56] 3[63] CAGCAGGCCGAGGTGCGGGCGCTA
1[90] 3[95] AAATCGTGAACCATCGCTGCGC
1[115] 3[127] AAAAGAATAGCCCAAAACCGTAATGCGCC
2[47] 4[32] ACTAAATCGGAACCCTCAAGCGGTAAGAGTCT
2[79] 4[64] TCAAGTTTTTTGGGGTGAAAATCCGTGTTTTT
2[111] 4[96] GCGATGGCCCACTACGGCAAAATCTAGACAGG
2[143] 4[128] CAACGTCAAAGGGCGAGAGATAGGGGAGCTAA
3[24] 8[32] GCGAGAAAGGAAGGGATAGTCTTTCTTGCCTGGACTTTAC
3[64] 8[64] GGGCGCTGACGTGGCACTTGCTGGTAGATAAT
3[96] 8[96] GTAACCACATAGAACCCAGCCATTAGCACTAA
3[128] 8[128] GCTACAGGCAGTAATAATACCTACAATTGAGG
3[144] 0[144] TATGGTTGCTTAACGTGGACTCTTGTTCCAGTTCGTTGCGCTCA
4[31] 7[31] GTCCATCATAACATCAAATGCGCGGAACCACC
4[63] 7[63] ATAATCAGCTATCGGCCAGACAATGCGGTCAG
4[79] 0[65] CCTGAGAATGTTTGATGGTCGGGGAGAGGCGGTTTGC
4[95] 7[95] AACGGTACATTACCGCCTTCTGACCACGCTGA
4[111] 0[97] AGGGATTTCCTTATAAATCGTGCCAGCTGCATTAATG
4[127] 7[127] ACAGGAGGTCATGGAAAAAGGGACAAAGCATC
4[154] 6[144] CCTCGTTAGAAGCTCAATCGTCGATTCACCAGT
5[16] 8[16] ATTAGTAAAACTGATATACCGAACCGACAACT
5[48] 2[48] AACTCAAATGAGGCCAAAAGGAGCCGTAAAGC
5[80] 2[80] AGAACAATGCCAGAATAGCGGTCACACCCAAA
5[112] 2[112] AAAAACGCCCGATTAACCGCGCTTCTATCAGG
6[47] 0[33] ATGGCTATAGAAAGCGCCGAGTAACCACGCTGGGTTTTTCTTTTCAC
6[143] 0[129] CACACGACGCGCGTACTCAGAGCGGTTGAGTGCTGCCCGCTTTCCAG
7[32] 5[47] AGCAGAAGATAAAACAAAGTATTAAGTAGAAG
7[64] 5[79] TATTAACACCGCCTGCGCCGTCAATAATATCC
7[96] 5[111] GAGCCAGCAGCAAATGTCTTTAGGGCAACAGG
7[128] 5[143] ACCTTGCTGAACCTCATTGAAAGGATTTTGAC
8[31] 10[40] AAACAATTTTTAAAAGTTTGAGTAACATTATCGAAACAAT
8[63] 10[72] ACATTTGAGAACAAAGAAACCACCAGAAGGAGAATATACA
8[87] 3[79] AGATTAGAAACAGTGCCTGAAAGCGTAAGAATGCAAGTGT
8[95] 10[104] CAACTAATTCATCATATTCCTGATTATCAGATATTGCGTA
8[119] 3[111] CTAAAATAAAAAATCTATTCTGGCCAACAGAGCACACCCG
8[127] 10[136] AAGGTTATTTCATCAATATAATCCTGATTGTTAAATTATT
9[16] 11[23] TTATTAATTGCTTTGAAGGCGAAT
9[48] 6[48] ATTTTGCGGGATTTAGGAGGTGAGATTTTTGA
9[144] 7[154] TGGATTATACTCAAATCAACAGAATATCAAACC
10[39] 15[39] AACGGATTCAAGACAATTTTTAACAGACGACG
10[71] 15[71] GTAACAGTTATATTTTATTTATCATGCAGAAC
10[103] 15[103] GATTTTCAATTTAATGTAGATTAAAGTCCTGA
10[135] 15[143] TGCACGTAAATAAGGCCCCTTAGAAATTTACGAGCATGTA
11[40] 14[40] CTGAGCAAAGACTACCAGAACGCGTTAGGCAG
11[72] 14[72] AGAAAACAAATAGTGAAGTTAATTAATCGCCA
11[104] 14[104] ATTTGAATCGATAGCTGTTTGAAAAAGCCAAC
11[136] 14[136] ATAAATCATTAATTTTGTTAAATATCATATGC
12[55] 10[48] GGTCTGAGAAGAAGATCATCGGGA
12[87] 9[87] GAAGAGTCAAATTAATGTCAGATGCGGAATTA
12[119] 9[119] AAACATAGTACCTTTTATAAAGAAGATGGCAA
13[24] 11[39] TCCAATCGCGCCTGATTATTCATTTCAATTAC
13[56] 11[71] TTTTCAAAACCTTTTAGATGAAACAAACATCA
13[88] 11[103] TGACCTAAGGTTTAACTACATTTAACAATTTC
13[120] 11[135] GTGTGATAAAACAGAATTAATGGAAACAGTAC
13[144] 10[144] AGAATAAACACGTCGCTATTAAATATATGTGAGCTACCATATCA
14[39] 16[32] AGGCATTTAAGCCTTAGCTTATCC
14[71] 16[56] TATTTAACACCCAGCTGCGCCCAATAGCAAGC
14[103] 16[88] GCTCAACAACGCTAACCCGTTTTTATTTTCAT
14[135] 16[120] GTTATACAGCCAGTTAACCAAGTACCGCACTC
15[40] 19[47] ACAATAAATATAGAAGAATCAAGAAAGTCAGACAATGAAA
15[72] 17[79] GCGCCTGTTCATTACCACAATTTT
15[104] 18[104] ACAAGAAACAAGCAAGGAGCGTCTAGAGAGAA
16[31] 13[23] GGTATTCTAAGAACGCTAAAGTAAGTAATAAGGATGCAAA
16[55] 13[55] AAATCAGACAACATGTCATGTAATAGAAAACT
16[87] 13[87] CGTAGGAATTATCAACTAATTGAGTCATCTTC
16[119] 13[119] ATCGAGAAAATAATATCCAGTATATACCGACC
16[143] 19[143] GGTATTAACAAAATAATTAACGTCTAATAACG
17[24] 12[16] AGGTTTTGTCGAGCCATTCTGTCCCTCCGGCTTAGGTTGG
17[56] 12[56] TATTTTGCAACGCCAATCAGCTAAAAATCATA
17[88] 12[88] TCTTACCAGTAGGGCTAATAGATAGACGCTGA
17[120] 12[120] CCTAATTTAATTCTTACCCATCCTATCCTTGA
17[144] 14[144] ACAGCCATATTTTCCAAGAACGGAAACCAATCACCTGTTTAGTA
18[79] 21[79] TAGACGGGCCTTTTTACACGGAATCACCGACT
19[16] 21[23] AAGCCCAACCAAAGACAGGGAGGG
19[32] 17[23] GCAAGAAAGGGTAATTGAGCGCTAATATCAGACTTGCGGG
19[64] 17[55] ACCGAAGCAGAATTAACTGAACACCCTGAACATTAGTTGC
19[96] 17[87] AGCCGAACTAACATAAAAACAGGGAAGCGCATATCCTGAA
19[104] 23[119] AAAGTTACGGTGGCAAGCAAAATCACCAGTAGCTTTTGATCAGACGAT
19[128] 17[119] AAACGCAAAAAAATGAAAATAGCAGCCTTTACTTCCAGAG
20[47] 23[55] GAAAATTCGAAATTATTCATTAAAGCCCGTATTCAGAGCC
20[63] 22[48] TTTGTCACAATCAATATAGCAATATTTTCATA
20[87] 24[72] AAAGACACAGAAAAGTCGGTCATAGCCCCCTTCCAGAGCCGGGGTCAG
20[127] 22[112] AAAATACATACATAAACAGAAGGAGTCAGACT
20[143] 22[144] ATGTTAGCAAGGCCGGAAACGTCACCATAATCAGTAGC
20[154] 18[144] TATTACGCAGTGAATACCCAAACGATTTTTTGT
21[32] 20[24] TATTGACGATATGGTTTACCAGCG
21[80] 23[87] TGAGCCATGTTTTAACGCCGCCAG
22[23] 25[23] ACCACCGGCCTCAGAATTCGGAACTTAAGAGG
22[47] 25[47] ATCAAAATCACCACCCAAACAGTTGAAGGATT
22[111] 24[104] GTAGCGCGAGGCAGGTGATACAGG
22[143] 24[136] GACAGAATAAACAAATCGTTCCAG
23[24] 22[32] CCGCCACCCTCAGAGCCACCGGAA
23[56] 20[64] GCCACCAGAACCACCAATTAGCGTATCACCGTAAGTTTAT
23[88] 20[88] CATTGACAGGAGGTTGTTTTCATCTAGAGCCACATATAAAAGAAACGC
23[120] 20[128] TGGCCTTGATATTCACCAAGTTTGCCATTAGCAAACGTAG
23[144] 26[144] AAATCCTCATTTCTGAATTTACAGGTTTAGTACCAGAACCGCCA
25[24] 26[16] CTGAGACTGACAGCCCTCATAGTT
25[32] 19[31] CCTCAAGAAATGCCCCCTGCCTATAAGGTAAACCAGAGCCTAATAAGA
25[64] 19[63] TGCTCAGTTGCCTTGAGTAACAGTGGTGAATTTTGCCATCGCTATCTT
25[72] 1[55] ACCAGGCGACTGAGTTTCGTCACCGTATTGGGCGCCAGGGTGTTTGCCC
25[96] 19[95] AGGGTTGAAGTGTACTGGTAATAATTGGGAATGGCATTTTAAGCAGAT
25[104] 1[89] TATAAGTAAGCAAGCCCAATAGGAAATCGGCCAACGCGGGTTCCG
25[128] 19[127] TATCACCGTAAGCGTCATACATGGCACCATTACCTTTAGCAACCGAGG
25[136] 0[115] TACTCAGGCCCTCAGAGCCACCACTCGGGAAACCTGTC
26[47] 1[23] CCTGTAGCATTCCACACAGTGAGACGGGCAACACCTGAGAG
26[63] 25[63] AGTACAAACTACAACGAGGATTAGCGGGGTTT
26[95] 25[95] ACCCATGTACCGTAACGATAAGTGCCGTCGAG
26[127] 25[127] CCTCATTTTCAGGGATTAGCCCGGAATAGGTG


Staple of 5081NT
Start End Sequence
1[24] 4[16] TTGAATTATTCAAATATATTTTAGTTAATGGTCAGAACGC
1[56] 3[63] AAATCAATCAATCGCATAAATAAG
1[90] 3[95] GTAAATTATATAACTAGAAAAA
1[115] 3[127] TCCCTTAGAATCCACTACCTTTACAAATT
2[47] 4[32] AACGCGAGAAAACTTTCCTTTTTTACATGTTC
2[79] 4[64] AATGCTGATGCAAATCATATGTGAAAGTAATT
2[111] 4[96] CCGGCTTAGGTTGGGTCGTCGCTAAATAAGAG
2[143] 4[128] ATCATAGGTCTGAGAGTTGAAAACTGTAATTT
3[24] 8[32] TTGAAATACCGACCGTAGTTGCTATAATATCCGCAATAGC
3[64] 8[64] AATAAACATGCGGGAGAACCAATCGCCCAATA
3[96] 8[96] GCCTGTTTGAACGCGAATTCCAAGATCAGAGA
3[128] 8[128] CTTACCAGATCAGATAACTCATCGTGAACAAA
3[144] 0[144] CAACGCTCAACAATTTATCAAAAGCTTAGATTAAGTAACAGTAC
4[31] 7[31] AGCTAATGAAGAAAAATTTTGCACGCGTCTTT
4[63] 7[63] CTGTCCAGCATGTAGAGTTTTGAAAAATAAAC
4[79] 0[65] AAAAGGTAGTGAATAACCTATCGCGCAGAGGCGAATT
4[95] 7[95] AATATAAATCCTTATCGGCGTTTTAATAAGAA
4[111] 0[97] GAGCCAGTTTAATTAATTTTCGCCTGATTGCTTTGAA
4[127] 7[127] AGGCAGAGAGTACCGCTAGAAGGCATGAAAAT
4[154] 6[144] ATATTTAACAACAAGCCGTTTTCGCGCCCAATA
5[16] 8[16] TCCTGAACCCAGCTACGCTAACGACGAAGCCC
5[48] 2[48] TTTACGAGACGACGACTAAGGCGTAGACAAAG
5[80] 2[80] GCTGTCTTGTACCGACATAATTACTATATGTA
5[112] 2[112] TTAAACCAGCATTTTCATGCGTTATTTAACCT
6[47] 0[33] TCAAGATTGTGATAAAAATAAACAAATGGAAAGAGCAAAAGAAGATG
6[143] 0[129] GCAAGCAATATAAAGCCGCCAACAATAGCGATCTTTTACATCGGGAG
7[32] 5[47] CCAGAGCCTAATTTGCATGAAATACATCCTAA
7[64] 5[79] AGCCATATTATTTATCTGAGTTAAAATAATCG
7[96] 5[111] ACGATTTTTTGTTTAAGCGCTAATAACGGGTA
7[128] 5[143] AGCAGCCTTTACAGAGGAACACCCAGAACAAG
8[31] 10[40] TATCTTACAGTTACCAGAAGGAAACCGAGGAAAATTATTC
8[63] 10[72] ATAAGAGCATAACGGAATACCCAAAAGAACTGCGATTGAG
8[87] 3[79] ACAAGAATCCAATCCAAGCGAACCTCCCGACTCCGGAATC
8[95] 10[104] GATAACCCAAGACTCCTTATTACGCAGTATGTCCAGCGCC
8[119] 3[111] GTAATTGACGTCAAAATTATCCGGTATTCTAAAGTATCAT
8[127] 10[136] GTCAGAGGGTAGAAAATACATACATAAAGGTGTGTCACAA
9[16] 11[23] CCGAACAACACCGTCAGAGCCAGC
9[48] 6[48] ACGCAATAAAGAAACACAGTTACAGCCTTAAA
9[144] 7[154] GCAACATATAAGAGAATTAACTAGAATAACATA
10[39] 15[39] ATTAAAGGGACGATTGAGAGCCGCGCCCGGAA
10[71] 15[71] GGAGGGAAATCCTCATTCAGAACCGTTTAGTA
10[103] 15[103] AAAGACAACTCTGAATCACCGGAATCAGAACC
10[135] 15[143] TCAATAGAATGGCTTTTTCATAATATTTTCAGGGATAGCA
11[40] 14[40] CCATTACCCCACCCTCGCCTTGATAGGATTAG
11[72] 14[72] AATGAAACCGCCACCCTAAAGCCAAAAGTATT
11[104] 14[104] TAGCGACAAGAGCCACTTACCGTTGCCTATTT
11[136] 14[136] GACTGTAGGCCATCTTTGATGATAAACAGTGC
12[55] 10[48] CAGAGCCAATTAGCAATTGACGGA
12[87] 9[87] CTCAGAGCCATCGATACATTCAACGCATGATT
12[119] 9[119] CCGGAACCGAATCAAGATGGTTTATAGCAAAC
13[24] 11[39] GCAGGTCATGAATTATAAAATCACCAGTAGCA
13[56] 11[71] ACAAATAAGGTAAATAGGCCGGAAACGTCACC
13[88] 11[103] AGCGCAGTAAGGGCGAGCAGCACCGTAATCAG
13[120] 11[135] CGTCATACAAATTCATTTTGCCTTTAGCGTCA
13[144] 10[144] CAGGAGTGTACTATTAGCGTTTCGCGTTTTCATTAAGTTTATTT
14[39] 16[32] GATTAGCGGAATTGCGTGGGATTT
14[71] 16[56] AAGAGGCTATCTCCAACTTTCCAGACGTTAGT
14[103] 16[88] CGGAACCTTAATTGTAATAGTTAGCGTAACGA
14[135] 16[120] CCGTATAAGTGAATTTACAACGCCTGTAGCAT
15[40] 19[47] TAGGTGTATTTCTGTAAATAATAAGAGGGTAGAATGCCAC
15[72] 17[79] CCGCCACCTTTGTCGTAAAAAAGG
15[104] 18[104] GCCACCCTCAGCCCTCTCGGTTTAGCAGGGAG
16[31] 13[23] TGCTAAACAACTTTCAGGTTGATACTCAGTACAGGTTGAG
16[55] 13[55] AAATGAATTCACCGTATCAAGAGAATTCACAA
16[87] 13[87] TCTAAAGTCTCAGAACGAAACATGGAATGGAA
16[119] 13[119] TCCACAGACAGAGCCATGCCCCCTCCAGTAAG
16[143] 19[143] TACAAACTCTTAAACACGCATAACCAAAGTAC
17[24] 12[16] AACTAAAGGGGTTTTGTAAGTATACACCAGAACCACCACC
17[56] 12[56] CGTTGAAAGAGACTCCCTCAGGAGGCCACCCT
17[88] 12[88] GGAGCCTTATTATTCTCGCCACCCCCGCCTCC
17[120] 12[120] CTTTCGAGACAGTTAACCACCCTCCAAAATCA
17[144] 14[144] GCTTGATACCGTTCGTCACCAGAGCCCAATAGGGTGCCTTGAGT
18[79] 21[79] TCACCCTCAAGAGGCACCAACTTTCCCTGACG
19[16] 21[23] TTCCATTAACCTTCATGATATTCA
19[32] 17[23] AAATACGTCAACGGCTACAGAGGCTTTGAGGAAAAGGAAC
19[64] 17[55] TAAAACGAAGCAGCGAAAGACAGCATCGGAACTTTTTTCA
19[96] 17[87] CACTCATCTTAAAGGCCGCTTTTGCGGGATCGCTCCAAAA
19[104] 23[119] TTTGACCCGCAGACGGTTGGGCTTGAGATGGTCAAAAATCGTTTAGAC
19[128] 17[119] GCGCGAAACGATATATTCGGTCGCTGAGGCTTTCAGCTTG
20[47] 23[55] CAGACCAGAACAAAGCTGCTCATTAAGAGGAATAAAAACC
20[63] 22[48] ACAGATGAACGGTGTATACGAAGGATACCACA
20[87] 24[72] CGAACTGAAAAGAATAAGGTAGAAAGATTCATTTTGCAAAAAAGCGGA
20[127] 22[112] TTAGCCGGAACGAGGCCCAGCGATAATAAAAC
20[143] 22[144] ACCTGCTCTCATTGTGAATTACCTTATTACCAGTCAGG
20[154] 18[144] CGAAATCCGCGAACGGAGATTTACCATCGCCCA
21[32] 20[24] ATCAACGTGCGCATAGGCTGGCTG
21[80] 23[87] AGAAACACTCAGAAGCAGAAGTTT
22[23] 25[23] CCAAAAGGCACTATCATTTTAATTGAAGCAAA
22[47] 25[47] TTCAACTAGACGACGAGCCCGAAATTAGAGAG
22[111] 24[104] GAACTAACAGTAAAATAGGTCTTT
22[143] 24[136] ACGTTGGGCTGCGGAACAGAAAAC
23[24] 22[32] TAACCCTCGTTTACCAATGCAGAT
23[56] 20[64] AAAATAGCGAGAGGCTCAGTTGAGAAGGCTTGGAAAGAGG
23[88] 20[88] TGCCAGAGGGGGTAATGGAACAACGTAGTAAATCAATCATAAGGGAAC
23[120] 20[128] TGGATAGCGTCCAATAAAGAAAAAAACTTTAACATGTTAC
23[144] 26[144] TCGTCATAAATTTTAAACAGTTTGCAACTAAAGATATAACAGTT
25[24] 26[16] CTCCAACACATCAATTCTACTAAT
25[32] 19[31] GGTCAGGAGACTTCAAATATCGCGTTACCCAAACATAACGAACGGGTA
25[64] 19[63] TTTTGATATTGCATCAAAAAGATTCAGTGAATATTTAGGACACCAACC
25[72] 1[55] AGAGGTCATAACCTGTTTAGCTATATTCATTTCAATTACCTCAGTACAT
25[96] 19[95] AGAGCTTAACCCTGACTATTATAGCAGAACGAATTATTACCACTAAAA
25[104] 1[89] ATTGCTGATTGACCATTAGATACATACCAAGTTACAAATGCTTCT
25[128] 19[127] CAACATGTGAGAATGACCATAAATTTAATTTCTCTACGTTTATACCAA
25[136] 0[115] TTTAAATAGATTCCCAATTCTGCGAAACAATAACGGAT
26[47] 1[23] CGAGCTGAAAAGGTGGATGAAACAAACATCAAGAATTTCAT
26[63] 25[63] ATTTTCATTTGGGGCGTACCTTTAATTGCTCC
26[95] 25[95] TTTCGCAAATGGTCAATTTTTGCGGATGGCTT
26[127] 25[127] AACGAGTAGATTTAGTATATAATGCTGTAGCT


Trials and errors

Initial design


▲Initial design of DNA silk

- Prepare ssDNA backbone via RCR. In it, red parts correspond to amorphous segments of spider silk and blue parts correspond to crystalline segments of spider silk.
- To turn DNA strands(blue parts) into a plate where DNA block would be attached, DNA oligos are added. Blue parts unconnected with oligos would form base pair with DNA origami block.
- Once DNA origami blocks are added, block-backbone structure is formed.

Idea to prevent gelation and bonding strands each other

Idea 1

- Connect Plates in each strands after reducing flexibility of DNA silk by attaching ssDNA oligomers
- Then detach ssDNA oligomers using ssDNA oligomers with fully complementary sequence

Idea 2

A segment : not attached to DNA origami plate, B segment : attached to DNA origami plate

- Oligomer has 1A'-2A'-3A' sequence in the ordered. In picture, ssDNA 1 from plasmid 1 has 1A-1B-1A-1B and ssDNA 2 from plasmid 2 has 2A-2B-2A-2B....
- By oligomers, each strand connects with one another

Idea 3
- Make it as fabric instead of thread. Electrospinning could be used for this process.

Others


Idea about crystalline segment

Structure of brick

1. All sides of trigonal prism are made from one scaffold.(composed of 1 strands.)
→
2. 3 sides made from other 3 scaffold with 1/3 scale.(composed of 3 strands.)
→

Idea 1-Rectangular plate(1)

6bp * 42 bp * 2layer = 504bp

Idea 2-Rectangular plate(2)

12bp * 42 bp * 2layer = 1008bp

Idea 3-Trigonal prism shaped brick(Honeycomb)


Idea 4-Trigonal prism shaped brick(Square)

About 880bp, height : 80bp

Idea 5-Thick Trigonal prism shaped brick

About 2080bp, height : 80bp

Composition of BCC(Brick-Connector Complex) or Brick complex

Brick(Red) : Connector(Blue) = 3 : 1


Brick(Red) : Connector(Blue) = 3 : 3


Brick A(Red) : Brick B(Blue) = 3 : 3


Connecting location on brick & connector

Interlocking staple         Picture of linking state             Linking state by side view

1.
2.
3.

Red : Brick, Blue : Connector, Yellow : linking staple

We marked where brick and connector are connected by staple(figures above). Scaffold was used to check stability through cando progrem.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=LabName:Supplementary&oldid=837337"