Mackenzie L. Cowell: added fluorescence wavelength info

2007-08-01T18:55:46Z

added fluorescence wavelength info

←Older revision		Revision as of 18:55, 1 August 2007
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	We added some red food coloring to all the dna we prepared for the iGEM 2007 part kits (1500 parts) to aid the process of visually confirming that 3uL had successfully been deposited in each well of each plate by our automation. Unfortunately, the presence of the dye seems to disrupt sequencing reactions.		We added some red food coloring to all the dna we prepared for the iGEM 2007 part kits (1500 parts) to aid the process of visually confirming that 3uL had successfully been deposited in each well of each plate by our automation. Unfortunately, the presence of the dye seems to disrupt sequencing reactions.

-	~~I will post some absorption and emission plots of~~ the ~~dye and the three others it came~~ with. The food coloring is from McCormick and the box is labeled ''Assorted Food Colors & Egg Dyes''. The main ingredients are: Water, Propylene Glycol, [http://en.wikipedia.org/wiki/FD%26C_Yellow_5 FD&C Yellow 5], [http://en.wikipedia.org/wiki/Allura_Red_AC FD&C Red 40], [http://en.wikipedia.org/wiki/Brilliant_Blue_FCF FD&C Blue 1], [http://en.wikipedia.org/wiki/FD%26C_Red_3 FD&C Red 3], and 0.1% Propylparaben.	+	Fluorometric analysis indicates the red food coloring fluoresces at 550 nm when illuminated with white light. The food coloring is from McCormick and the box is labeled ''Assorted Food Colors & Egg Dyes''. The main ingredients are: Water, Propylene Glycol, [http://en.wikipedia.org/wiki/FD%26C_Yellow_5 FD&C Yellow 5], [http://en.wikipedia.org/wiki/Allura_Red_AC FD&C Red 40], [http://en.wikipedia.org/wiki/Brilliant_Blue_FCF FD&C Blue 1], [http://en.wikipedia.org/wiki/FD%26C_Red_3 FD&C Red 3], and 0.1% Propylparaben.

Mackenzie L. Cowell: New page: This page is intended to serve as a centralized collection point for all those otherwise unwritten tips and tricks that we learn from more experienced labmates or from painful trial and er...

2007-07-03T00:36:20Z

New page: This page is intended to serve as a centralized collection point for all those otherwise unwritten tips and tricks that we learn from more experienced labmates or from painful trial and er...

New page

This page is intended to serve as a centralized collection point for all those otherwise unwritten tips and tricks that we learn from more experienced labmates or from painful trial and error.

=== Red Food Coloring: Is it Fluorescent? ===
We added some red food coloring to all the dna we prepared for the iGEM 2007 part kits (1500 parts) to aid the process of visually confirming that 3uL had successfully been deposited in each well of each plate by our automation. Unfortunately, the presence of the dye seems to disrupt sequencing reactions.

I will post some absorption and emission plots of the dye and the three others it came with. The food coloring is from McCormick and the box is labeled ''Assorted Food Colors & Egg Dyes''. The main ingredients are: Water, Propylene Glycol, [http://en.wikipedia.org/wiki/FD%26C_Yellow_5 FD&C Yellow 5], [http://en.wikipedia.org/wiki/Allura_Red_AC FD&C Red 40], [http://en.wikipedia.org/wiki/Brilliant_Blue_FCF FD&C Blue 1], [http://en.wikipedia.org/wiki/FD%26C_Red_3 FD&C Red 3], and 0.1% Propylparaben.

Lab Lore - Revision history

Mackenzie L. Cowell: added fluorescence wavelength info

Mackenzie L. Cowell: New page: This page is intended to serve as a centralized collection point for all those otherwise unwritten tips and tricks that we learn from more experienced labmates or from painful trial and er...