Laccase Protocols
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Introduction
This protocols are used to screent for and assay laccase activity.
Bromophenol Blue Plate Screen
Materials
Bromophenol blue
Procedure
Notes
References
Guiacol Colony Screen
Materials
- Gum Guiac or Guiacol
- 95% Ethanol
Procedure
1. Dissolve either 17mg gum guaiac or 13mg guaiacol into 1ml of 100% ethanol.
- If your guaiacol is in liquid form then add 12μL to 1ml ethanol.
2. Drop one drops of this solution on a large colony (2 days old for bacteria). 3. Wait four hours and a laccase positive colony should turn purple. 4. Bacteria don't usually like ethanol, so now they're probably dead.
Notes
References
Lignin Plate Screen
Materials
- Alkaline Lignin
- Ferric Chloride
- Potassium ferric cyanide
- Apropriate agar growth medium
Procedure
Notes
References
ABTS Assay
Materials
Procedure
Notes
References
DMP Assay
Materials
- DMP (2,6-dimethoxyphenol)
- DMS (sodium 2,2-dimethylsuccinate)
Procedure
1. Prepare DMS solution
- 30.8mg DMP (2mM)
- Centrifuge 1ml of overnight culture
Notes
References
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