Lactobacillus compatibility: Difference between revisions
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | ||
'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415'''] | |||
'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]] | |||
Revision as of 01:38, 29 October 2008
Lactobacillus delbruckii subsp. bulgaricus and its Compatible Plasmid(s)for Electrotransformation
- VI104 strain-
- -pLEM415 plasmid (derived from E. coli-L. reuteri) (10^3-10^4) transformants/ug
- - pX3 plasmid (from L. delbrueckii) (10^3)
- - pJK650 plasmid (from L. delbrueckii) (10^3)
- - pULP8 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
- - pNZ12 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
- - pG+ host 4 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
- - pGB305 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
- - pGT633 plasmid(from L. reuteri) (10) (low copy plasmid)
- - pCU1882 plasmid(from L. curvatus) (10) (low copy plasmid)
- ATCC 11842 strain –
- -pJK650 plasmid (2 x 10^3)
Lactobacillus delbruckii subsp. lactis and Compatible Plasmid(s) for Electrotransformation
- ATCC 10697 strain -
- -pLEM415 plasmid (10^3)
- -pJK650 plasmid (10^3)
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
*Derek Ju 04:38, 29 October 2008 (EDT):Looking for compatible plasmids?? Look here: pJK650 and pLEM415 *Derek Ju 04:38, 29 October 2008 (EDT):Looking for the transformation protocol? Look here: lactobacillus transformation
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
-
et al, Applied and Environmental Microbiology 2002
Contact
- Derek Ju (derekju [at] mit.edu)
or instead, discuss this protocol.