Lactobacillus compatibility

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Current revision (04:39, 29 October 2008) (view source)
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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#Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
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'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
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#Looking for the transformation protocol? Look here: [[lactobacillus transformation]]
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'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [['''lactobacillus transformation''']]

Current revision

Contents

Lactobacillus delbruckii subsp. bulgaricus and its Compatible Plasmid(s)for Electrotransformation

  • VI104 strain-
    • -pLEM415 plasmid (derived from E. coli-L. reuteri) (10^3-10^4) transformants/ug
    • - pX3 plasmid (from L. delbrueckii) (10^3)
    • - pJK650 plasmid (from L. delbrueckii) (10^3)
    • - pULP8 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pNZ12 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pG+ host 4 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pGB305 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pGT633 plasmid(from L. reuteri) (10) (low copy plasmid)
    • - pCU1882 plasmid(from L. curvatus) (10) (low copy plasmid)
  • ATCC 11842 strain –
    • -pJK650 plasmid (2 x 10^3)

Lactobacillus delbruckii subsp. lactis and Compatible Plasmid(s) for Electrotransformation

  • ATCC 10697 strain -
    • -pLEM415 plasmid (10^3)
    • -pJK650 plasmid (10^3)


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

*Derek Ju 04:38, 29 October 2008 (EDT):Looking for compatible plasmids?? Look here: pJK650 and pLEM415

*Derek Ju 04:38, 29 October 2008 (EDT):Looking for the transformation protocol? Look here: [[lactobacillus transformation]]


Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. et al, Applied and Environmental Microbiology 2002 [Serror]

Contact

  • Derek Ju (derekju [at] mit.edu)

or instead, discuss this protocol.

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