Lactobacillus compatibility

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Current revision (04:39, 29 October 2008) (view source)
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'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
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'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]
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'''*[[User:Derek Ju|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [['''lactobacillus transformation''']]

Current revision

Contents

Lactobacillus delbruckii subsp. bulgaricus and its Compatible Plasmid(s)for Electrotransformation

  • VI104 strain-
    • -pLEM415 plasmid (derived from E. coli-L. reuteri) (10^3-10^4) transformants/ug
    • - pX3 plasmid (from L. delbrueckii) (10^3)
    • - pJK650 plasmid (from L. delbrueckii) (10^3)
    • - pULP8 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pNZ12 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pG+ host 4 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pGB305 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
    • - pGT633 plasmid(from L. reuteri) (10) (low copy plasmid)
    • - pCU1882 plasmid(from L. curvatus) (10) (low copy plasmid)
  • ATCC 11842 strain –
    • -pJK650 plasmid (2 x 10^3)

Lactobacillus delbruckii subsp. lactis and Compatible Plasmid(s) for Electrotransformation

  • ATCC 10697 strain -
    • -pLEM415 plasmid (10^3)
    • -pJK650 plasmid (10^3)


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

*Derek Ju 04:38, 29 October 2008 (EDT):Looking for compatible plasmids?? Look here: pJK650 and pLEM415

*Derek Ju 04:38, 29 October 2008 (EDT):Looking for the transformation protocol? Look here: [[lactobacillus transformation]]


Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. et al, Applied and Environmental Microbiology 2002 [Serror]

Contact

  • Derek Ju (derekju [at] mit.edu)

or instead, discuss this protocol.

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