Lactobacillus compatibility - Revision history

Derek Ju: /* Notes */

Derek Ju — Wed, 29 Oct 2008 08:39:37 GMT

Notes

←Older revision		Revision as of 08:39, 29 October 2008
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	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']		'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']

-	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]	+	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [['''lactobacillus transformation''']]

Derek Ju: /* Notes */

Derek Ju — Wed, 29 Oct 2008 08:39:08 GMT

Notes

←Older revision		Revision as of 08:39, 29 October 2008
Line 24:		Line 24:

	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']		'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
		+
	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]		'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]

Derek Ju: /* Notes */

Derek Ju — Wed, 29 Oct 2008 08:38:57 GMT

Notes

←Older revision		Revision as of 08:38, 29 October 2008
Line 24:		Line 24:

	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']		'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
-
-
	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]		'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]

Derek Ju: /* Notes */

Derek Ju — Wed, 29 Oct 2008 08:38:43 GMT

Notes

←Older revision		Revision as of 08:38, 29 October 2008
Line 24:		Line 24:

	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']		'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
		+
		+
	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]		'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]

Derek Ju: /* Notes */

Derek Ju — Wed, 29 Oct 2008 08:38:25 GMT

Notes

←Older revision		Revision as of 08:38, 29 October 2008
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	Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!		Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

-	#Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']	+	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
-	#Looking for the transformation protocol? Look here: [[lactobacillus transformation]]	+	'''*[[User:Derek Ju\|Derek Ju]] 04:38, 29 October 2008 (EDT)''':Looking for the transformation protocol? Look here: [[lactobacillus transformation]]

Derek Ju: /* Notes */

Derek Ju — Wed, 29 Oct 2008 08:35:46 GMT

Notes

←Older revision		Revision as of 08:35, 29 October 2008
Line 24:		Line 24:

	#Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']		#Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
-		+	#Looking for the transformation protocol? Look here: [[lactobacillus transformation]]

Derek Ju: /* Lactobacillus delbruckii subsp. bulgaricus and its Compatible Plasmid(s)for Electrotransformation */

Derek Ju — Wed, 29 Oct 2008 08:35:04 GMT

Lactobacillus delbruckii subsp. bulgaricus and its Compatible Plasmid(s)for Electrotransformation

←Older revision		Revision as of 08:35, 29 October 2008
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	**- pGT633 plasmid(from L. reuteri) (10) (low copy plasmid)		**- pGT633 plasmid(from L. reuteri) (10) (low copy plasmid)
	**- pCU1882 plasmid(from L. curvatus) (10) (low copy plasmid)		**- pCU1882 plasmid(from L. curvatus) (10) (low copy plasmid)
-	*ATCC 11842 strain – pJK650 plasmid (2 x 10^3)	+	*ATCC 11842 strain –
		+	**-pJK650 plasmid (2 x 10^3)

	==''Lactobacillus delbruckii'' subsp. ''lactis'' and Compatible Plasmid(s) for Electrotransformation==		==''Lactobacillus delbruckii'' subsp. ''lactis'' and Compatible Plasmid(s) for Electrotransformation==

Derek Ju: /* Notes */

Derek Ju — Wed, 29 Oct 2008 08:34:38 GMT

Notes

←Older revision		Revision as of 08:34, 29 October 2008
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	==Notes==		==Notes==
	Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!		Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
-	#~~Where to find these~~ plasmids? ~~Find pJK650~~ [http://partsregistry.org/Part:BBa_K128008 ~~here~~] and ~~pLEM415~~ [http://partsregistry.org/Part:BBa_K128007 ~~here~~]	+
		+	#Looking for compatible plasmids?? Look here: [http://partsregistry.org/Part:BBa_K128008 '''pJK650'''] and [http://partsregistry.org/Part:BBa_K128007 '''pLEM415''']
		+
		+

	Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.		Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.

Derek Ju: New page: ==''Lactobacillus delbruckii'' subsp. ''bulgaricus'' and its Compatible Plasmid(s)for Electrotransformation== *VI104 strain- **-pLEM415 plasmid (derived from E. coli-L. reuteri) (10^3-10...

Derek Ju — Wed, 29 Oct 2008 08:26:25 GMT

New page: ==''Lactobacillus delbruckii'' subsp. ''bulgaricus'' and its Compatible Plasmid(s)for Electrotransformation== *VI104 strain- **-pLEM415 plasmid (derived from E. coli-L. reuteri) (10^3-10...

New page

==''Lactobacillus delbruckii'' subsp. ''bulgaricus'' and its Compatible Plasmid(s)for Electrotransformation==
*VI104 strain-
**-pLEM415 plasmid (derived from E. coli-L. reuteri) (10^3-10^4) transformants/ug
**- pX3 plasmid (from L. delbrueckii) (10^3)
**- pJK650 plasmid (from L. delbrueckii) (10^3)
**- pULP8 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
**- pNZ12 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
**- pG+ host 4 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
**- pGB305 plasmid (heterologous plasmid) (10^2) (low copy plasmid)
**- pGT633 plasmid(from L. reuteri) (10) (low copy plasmid)
**- pCU1882 plasmid(from L. curvatus) (10) (low copy plasmid)
*ATCC 11842 strain – pJK650 plasmid (2 x 10^3)

==''Lactobacillus delbruckii'' subsp. ''lactis'' and Compatible Plasmid(s) for Electrotransformation==
*ATCC 10697 strain -
**-pLEM415 plasmid (10^3)
**-pJK650 plasmid (10^3)

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
#Where to find these plasmids? Find pJK650 [http://partsregistry.org/Part:BBa_K128008 here] and pLEM415 [http://partsregistry.org/Part:BBa_K128007 here]

Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.

==References==
'''Relevant papers and books'''

<biblio>
#Serror et al, Applied and Environmental Microbiology 2002
</biblio>

==Contact==
*Derek Ju (derekju [at] mit.edu)

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].

[[Category:Lactobacillus]]