Lactobacillus miniprep

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Revision as of 18:17, 29 October 2008 by Derek Ju (talk | contribs) (New page: ==Overview== A miniprep for ''Lactobacillus delbruckii'' (development in progress) ==Procedure== #Incubate cells for 30-45 minutes in TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose) wit...)
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Overview

A miniprep for Lactobacillus delbruckii (development in progress)


Procedure

  1. Incubate cells for 30-45 minutes in TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose) with 10mg/ml lysozyme and 70U/ml mutanolysin.
  2. Perform normal QIAGEN miniprep
  3. verify extraction of plasmid by sequencing or digesting and running on gel

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books Serror et al, Applied and Environmental Microbiology, 2002

Contact

  • Derek Ju (derekju [at] mit.edu)

or instead, discuss this protocol.

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