Lactobacillus planarum miniprep
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(New page: ==Overview== Method to miniprep plasmid DNA from ''Lactobacillus plantarum'' ==Procedure== #Step 1 #Step 2 #*Step 2 has some additional information that goes with it. i.e. Keep at 4&d...) |
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==Procedure== | ==Procedure== | ||
| - | # | + | #Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase |
| - | # | + | #incubate at 37C for 15-30 minutes |
| - | + | #Perform QIAGEN miniprep | |
| - | # | + | #Verify plasmid existance by sequencing or running digests on gel |
| - | # | + | |
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==Notes== | ==Notes== | ||
Revision as of 21:20, 29 October 2008
Contents |
Overview
Method to miniprep plasmid DNA from Lactobacillus plantarum
Procedure
- Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase
- incubate at 37C for 15-30 minutes
- Perform QIAGEN miniprep
- Verify plasmid existance by sequencing or running digests on gel
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
Mathiesen et al, Journal of Applied Microbiology 2004
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
