Lactobacillus planarum miniprep
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Revision as of 13:12, 23 February 2009
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Method to miniprep plasmid DNA from Lactobacillus plantarum
- Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase
- incubate at 37C for 15-30 minutes
- Perform QIAGEN miniprep
- Verify plasmid existance by sequencing or running digests on gel
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Relevant papers and books
Mathiesen et al, Journal of Applied Microbiology 2004
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