Lactobacillus planarum miniprep

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(Procedure)
Current revision (14:49, 7 May 2010) (view source)
(Procedure)
 
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{{back to protocols}}
==Overview==
==Overview==
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==Procedure==
==Procedure==
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#Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase  
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#Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
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#incubate at 37C for 15-30 minutes
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#incubate at 37°C for 15-30 minutes.
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#Perform QIAGEN miniprep  
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#Add Qiagen P2 buffer and continue miniprep as usual.
#Verify plasmid existance by sequencing or running digests on gel
#Verify plasmid existance by sequencing or running digests on gel
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'''Relevant papers and books'''
'''Relevant papers and books'''
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Mathiesen et al, Journal of Applied Microbiology 2004
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Mathiesen, G., H. M. Namlos, et al. (2004). "Use of bacteriocin promoters for gene expression in Lactobacillus plantarum C11." Journal of Applied Microbiology 96(4): 819-827.
==Contact==
==Contact==

Current revision

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Contents

Overview

Method to miniprep plasmid DNA from Lactobacillus plantarum


Procedure

  1. Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
  2. incubate at 37°C for 15-30 minutes.
  3. Add Qiagen P2 buffer and continue miniprep as usual.
  4. Verify plasmid existance by sequencing or running digests on gel

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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References

Relevant papers and books

Mathiesen, G., H. M. Namlos, et al. (2004). "Use of bacteriocin promoters for gene expression in Lactobacillus plantarum C11." Journal of Applied Microbiology 96(4): 819-827.

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.

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