Lactobacillus planarum miniprep: Difference between revisions
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==Overview== | ==Overview== | ||
Revision as of 10:12, 23 February 2009
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Overview
Method to miniprep plasmid DNA from Lactobacillus plantarum
Procedure
- Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase
- incubate at 37C for 15-30 minutes
- Perform QIAGEN miniprep
- Verify plasmid existance by sequencing or running digests on gel
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
Mathiesen et al, Journal of Applied Microbiology 2004
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
