Lactobacillus planarum miniprep

From OpenWetWare

Revision as of 15:49, 7 May 2010 by Michael A. Speer (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
back to protocols

Contents

Overview

Method to miniprep plasmid DNA from Lactobacillus plantarum


Procedure

  1. Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
  2. incubate at 37°C for 15-30 minutes.
  3. Add Qiagen P2 buffer and continue miniprep as usual.
  4. Verify plasmid existance by sequencing or running digests on gel

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

Mathiesen, G., H. M. Namlos, et al. (2004). "Use of bacteriocin promoters for gene expression in Lactobacillus plantarum C11." Journal of Applied Microbiology 96(4): 819-827.

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.

Personal tools