Lactobacillus transformation (Speer 2012): Difference between revisions
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*Glycine | *Glycine | ||
*Milipore water | *Milipore water | ||
*50mM EDTA solution | |||
*0.5M Sucrose, 10% glycerol solution | |||
==Procedure== | ==Procedure== | ||
Revision as of 12:21, 14 September 2011
Back to Protocols
Back to Electro-transformation of Lactobacillus spp.
Overview
This is basically a modified version of the Mason (2005 protocol) for use with Lactobacillus plantarum and an Eppendorf 2510 electroporator.
Materials
- MRS media
- Glycine
- Milipore water
- 50mM EDTA solution
- 0.5M Sucrose, 10% glycerol solution
Procedure
Notes
All questions, input and feedback are welcome!
References
There will be one here soon.
Contact
- mas853@psu.edu
or instead, discuss this protocol. -->
