Lactobacillus transformation (Speer 2012)

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(Materials)
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*Glycine
*Glycine
*Milipore water
*Milipore water
-
 
+
*50mM EDTA solution
 +
*0.5M Sucrose, 10% glycerol solution
==Procedure==
==Procedure==

Revision as of 14:21, 14 September 2011

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Back to Electro-transformation of Lactobacillus spp.

Contents

Overview

This is basically a modified version of the Mason (2005 protocol) for use with Lactobacillus plantarum and an Eppendorf 2510 electroporator.

Materials

  • MRS media
  • Glycine
  • Milipore water
  • 50mM EDTA solution
  • 0.5M Sucrose, 10% glycerol solution

Procedure

Notes

All questions, input and feedback are welcome!

References

There will be one here soon.


Contact

  • mas853@psu.edu

or instead, discuss this protocol. -->

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