Lactobacillus transformation (Speer 2012)
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Revision as of 15:40, 14 September 2011
This is basically a modified version of the Mason (2005 protocol) for use with Lactobacillus plantarum and an Eppendorf 2510 electroporator.
- MRS media
- Milipore water
- 50mM EDTA solution
- 0.5M Sucrose, 10% glycerol solution
- Grow L. plantarum overnight from freezer stock in 5ml MRS medium (30°C without shaking).
- Add 1g glycine to two flasks of 50ml MRS medium to make 100ml 2% glycine.
- Shake to dissolve.
- Add 1ml overnight culture to each flask (1:50 dlution)
- Culture for ~3 hours.
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