Lan:LE daily entries: Difference between revisions
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*'''Experimental procedures:''' Refer to appropriate protocol and note any changes in them | *'''Experimental procedures:''' Refer to appropriate protocol and note any changes in them | ||
*'''Results:''' Post pictures with caption and appropriate identification of lanes on gels | *'''Results:''' Post pictures with caption and appropriate identification of lanes on gels | ||
State what are your finding and any comments | **State what are your finding and any comments | ||
<h1>2008/06/30 - AP06-39</h1> | <h1>2008/06/30 - AP06-39</h1> | ||
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Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression | Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression | ||
<h3>Experimental procedures</h3> | <h3>Experimental procedures</h3> | ||
* Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL)to OD~0.4 | * Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL) to OD~0.4 | ||
* Induced with 0.1 mM IPTG (100 µL of 100 mM stock) | * Induced with 0.1 mM IPTG (100 µL of 100 mM stock) | ||
* Incubated in shaker B at 37°C O/N | * Incubated in shaker B at 37°C O/N | ||
* Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else) | * Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else) | ||
<h3>Results</h3> | <h3>Results</h3> | ||
Revision as of 06:51, 29 June 2008
Please follow this format for your entries
- Date(year/month/day)-Hard copy lab book reference (Initials lab book #-page)
- Title of experiment with brief description
- Experimental procedures: Refer to appropriate protocol and note any changes in them
- Results: Post pictures with caption and appropriate identification of lanes on gels
- State what are your finding and any comments
2008/06/30 - AP06-39
Expression assay on positives clones
Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression
Experimental procedures
- Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL) to OD~0.4
- Induced with 0.1 mM IPTG (100 µL of 100 mM stock)
- Incubated in shaker B at 37°C O/N
- Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else)
