Lan:LE daily entries: Difference between revisions
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<caption>SDS-PAGE 15% 200V 45 min</caption> | <caption> SDS-PAGE 15% 200V 45 min </caption> | ||
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<td> 8 µL </td> | <td> 8 µL </td> | ||
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Revision as of 09:29, 29 June 2008
Please follow this format for your entries
- Date(year/month/day)-Hard copy lab book reference (Initials lab book #-page)
- Title of experiment with brief description
- Experimental procedures: Refer to appropriate protocol and note any changes in them
- Results: Post pictures with caption and appropriate identification of lanes on gels
- State what are your finding and any comments
2008/06/30 - AP06-39
Expression assay on positives clones
Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression
Experimental procedures
- Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL) to OD~0.4
- Induced with 0.1 mM IPTG (100 µL of 100 mM stock)
- Incubated in shaker B at 37°C O/N
- Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else)
Results
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| Lane | Sample | Volume |
|---|---|---|
| 1 | Marker PS | 8 µL |
