Lan:LE daily entries: Difference between revisions
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Please follow this format for your entries | Please follow this format for your entries | ||
'''Date'''(year/month/day)-Hard copy lab book reference (Initials lab book #-page) | |||
'''Title of experiment with brief description''' | |||
'''Experimental procedures:''' Refer to appropriate protocol and note any changes in them | |||
'''Results:''' Post pictures with caption and appropriate identification of lanes on gels | |||
State what are your finding and any comments | |||
<h1>2008/06/30 - AP06-39</h1> | <h1>2008/06/30 - AP06-39</h1> | ||
<h2>Expression assay on positives clones</h2> | <h2>Expression assay on positives clones</h2> | ||
Digestion positives clone(ND01-XX) where tested for protein expression | Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression | ||
<h3>Experimental procedures</h3> | <h3>Experimental procedures</h3> | ||
* Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL)to OD~0.4 | |||
* Induced with 0.1 mM IPTG (100 µL of 100 mM stock) | |||
* Incubated in shaker B at 37°C O/N | |||
* Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else) | |||
<h3>Results</h3> | <h3>Results</h3> | ||
Revision as of 06:49, 29 June 2008
Please follow this format for your entries Date(year/month/day)-Hard copy lab book reference (Initials lab book #-page) Title of experiment with brief description Experimental procedures: Refer to appropriate protocol and note any changes in them Results: Post pictures with caption and appropriate identification of lanes on gels State what are your finding and any comments
2008/06/30 - AP06-39
Expression assay on positives clones
Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression
Experimental procedures
- Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL)to OD~0.4
- Induced with 0.1 mM IPTG (100 µL of 100 mM stock)
- Incubated in shaker B at 37°C O/N
- Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else)
