Lan:LE daily entries

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<caption> SDS-PAGE 15% 200V 45 min </caption>
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  <td> Vol µL </td>
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Revision as of 12:41, 29 June 2008


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Lab note books

Please follow this format for your entries

  • Date(year/month/day)-Hard copy lab book reference (Initials lab book #-page)
  • Title of experiment with brief description
  • Experimental procedures: Refer to appropriate protocol and note any changes in them
  • Results: Post pictures with caption and appropriate identification of lanes on gels
    • State what are your finding and any comments


2008/06/30 - AP06-39

Expression assay on positives clones

Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression

Experimental procedures

  • Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL) to OD~0.4
  • Induced with 0.1 mM IPTG (100 µL of 100 mM stock)
  • Incubated in shaker B at 37°C O/N
  • Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else)



SDS-PAGE 15% 200V 45 min
Lane Sample Volume
1 Marker PS 8 µL
2 Sample Vol µL
3 Sample Vol µL
4 Sample Vol µL
5 Sample Vol µL
6 Sample Vol µL
7 Sample Vol µL
8 Sample Vol µL
9 Sample Vol µL
10 Sample Vol µL
11 Sample Vol µL
12 Sample Vol µL
13 Sample Vol µL
14 Sample Vol µL
15 Sample Vol µL

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