Lan:LE daily entries: Difference between revisions
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<h3>Results</h3> | <h3>Results</h3> | ||
<span>[[Image: | <span>[[Image:AP06-52.jpg]] | ||
<table border="1"> | <table border="1"> | ||
6045 min </caption> | |||
<!-- This is the table header --> | <!-- This is the table header --> | ||
<tr> | <tr> | ||
| Line 30: | Line 30: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
<td> | <td> A4-S PS</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>2</td> | <td>2</td> | ||
<td> | <td> A9-S</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>3</td> | <td>3</td> | ||
<td> | <td> A21-L</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>4</td> | <td>4</td> | ||
<td> | <td> A22-L</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>5</td> | <td>5</td> | ||
<td> | <td> B3-S</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>6</td> | <td>6</td> | ||
<td> | <td> B12-L</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>7</td> | <td>7</td> | ||
<td> | <td> B16-L</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>8</td> | <td>8</td> | ||
<td> | <td> C32-S</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>9</td> | <td>9</td> | ||
<td> | <td> C33-S</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>10</td> | <td>10</td> | ||
<td> | <td> C34-S</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>11</td> | <td>11</td> | ||
<td> | <td> C36-S</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>12</td> | <td>12</td> | ||
<td> | <td> C7-S</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>13</td> | <td>13</td> | ||
<td> | <td> C42-L</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>14</td> | <td>14</td> | ||
<td> | <td> C44-L</td> | ||
<td> | <td> 10 µL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>15</td> | <td>15</td> | ||
<td> | <td> PS MARKER</td> | ||
<td> | <td> 8µL </td> | ||
</tr> | </tr> | ||
</span> | |||
Revision as of 06:04, 2 July 2008
Please follow this format for your entries
- Date(year/month/day)-Hard copy lab book reference (Initials lab book #-page)
- Title of experiment with brief description
- Experimental procedures: Refer to appropriate protocol and note any changes in them
- Results: Post pictures with caption and appropriate identification of lanes on gels
- State what are your finding and any comments
2008/06/30 - AP06-39
Expression assay on positives clones
Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression
Experimental procedures
- Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL) to OD~0.4
- Induced with 0.1 mM IPTG (100 µL of 100 mM stock)
- Incubated in shaker B at 37°C O/N
- Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else)
Results
| Lane | Sample | Volume |
|---|---|---|
| 1 | A4-S PS | 10 µL |
| 2 | A9-S | 10 µL |
| 3 | A21-L | 10 µL |
| 4 | A22-L | 10 µL |
| 5 | B3-S | 10 µL |
| 6 | B12-L | 10 µL |
| 7 | B16-L | 10 µL |
| 8 | C32-S | 10 µL |
| 9 | C33-S | 10 µL |
| 10 | C34-S | 10 µL |
| 11 | C36-S | 10 µL |
| 12 | C7-S | 10 µL |
| 13 | C42-L | 10 µL |
| 14 | C44-L | 10 µL |
| 15 | PS MARKER | 8µL |
