Lan:LE daily entries

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Revision as of 09:05, 2 July 2008

Image:Lan_yellow8x3.png‎

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Lab note books

Please follow this format for your entries

  • Date(year/month/day)-Hard copy lab book reference (Initials lab book #-page)
  • Title of experiment with brief description
  • Experimental procedures: Refer to appropriate protocol and note any changes in them
  • Results: Post pictures with caption and appropriate identification of lanes on gels
    • State what are your finding and any comments

Contents

2008/06/30 - AP06-39

Expression assay on positives clones

Digestion positives clone(ND01-XX and ND01-YY) where tested for protein expression

Experimental procedures

  • Used O/N culture for miniprep to inoculate 10 mL 2XYTGB flasks(125 mL) to OD~0.4
  • Induced with 0.1 mM IPTG (100 µL of 100 mM stock)
  • Incubated in shaker B at 37°C O/N
  • Harvested at 7:30 and lysed using lysis buffer (10X stock diluted to 1X, nothing else)

Results

Image:AP06-52.jpg‎

SDS-PAGE 15% 60 min
Lane Sample Volume
1 A4-S PS 10 µL
2 A9-S 10 µL
3 A21-L 10 µL
4 A22-L 10 µL
5 B3-S 10 µL
6 B12-L 10 µL
7 B16-L 10 µL
8 C32-S 10 µL
9 C33-S 10 µL
10 C34-S 10 µL
11 C36-S 10 µL
12 C7-S 10 µL
13 C42-L 10 µL
14 C44-L 10 µL
15 PS MARKER 8µL
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