Lane:MagneticPlasmidPrep

Overview

This is a work-in-progress protocol with the goal of replacing silica columns for bacterial minipreps with a magnetic bead based protocol. The starting point is ref. [1].

Materials

For enough beads for

• ingredient 1

Procedure

Preparing magnetic bead stock Carboxy-coated magnetic beads are Sera-mag SpeedBeads (Fisher #09-981-123). These come at 50mg/ml; protocol calls for 20mg/ml in 0.5M EDTA pH7.2:

1. Mix 400µl Sera-mag stock and 600µl ddH₂O
2. Collect beads on magnet.
3. Remove supernatant
4. Resuspend beads in 1ml 0.5M EDTA pH 7.2.
5. Place tube back on magnet. Remove supe.
6. Resuspend beads in 1ml 0.5M EDTA pH 7.2 again.

Store at 4˚C.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. This is an untested work in progress.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |

Contact

• Who has experience with this protocol?

or instead, discuss this protocol.