Laub:PYE: Difference between revisions
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*10mL 50 mM CaCl<sub>2</sub> | *10mL 50 mM CaCl<sub>2</sub> | ||
**From Emanuele: not necessary for Caulobacter growth at MIT (perhaps enough calcium already in yeast extract or kitchen flasks). | **From Emanuele: not necessary for Caulobacter growth at MIT (perhaps enough calcium already in yeast extract or kitchen flasks). | ||
**Now we use 1mL of 0.5M CaCl<sub>2</sub> from the MIT Kitchen --[[Sri Kosuri]] ([[User talk:Skosuri|talk]]) 16:49, 19 April 2007 (EDT) | |||
*1L H<sub>2</sub>O (milliQ or distilled) | *1L H<sub>2</sub>O (milliQ or distilled) | ||
Latest revision as of 13:49, 19 April 2007
Rich Media for Caulobacter crescentus
Ingredients
- 2.0g bactopeptone
- 1.0g yeast extract
- 0.3g MgSO4
- From Sigma
- 10mL 50 mM CaCl2
- From Emanuele: not necessary for Caulobacter growth at MIT (perhaps enough calcium already in yeast extract or kitchen flasks).
- Now we use 1mL of 0.5M CaCl2 from the MIT Kitchen --Sri Kosuri (talk) 16:49, 19 April 2007 (EDT)
- 1L H2O (milliQ or distilled)
Instructions
- Add ingredients and autoclave (250°F, 22psi, 30-60min depending on volume).
- For plates, usually add 1.5% (w/v) BactoAgar as well.
Notes
- If PYE is to be aliquoted, first prepare 1L as described, then aliquot/pour into 100mL bottles. Finally, reautoclave individual bottles
