Laub:PYE

Rich Media for Caulobacter crescentus

Ingredients

• 2.0g bactopeptone
• 1.0g yeast extract
• 0.3g MgSO₄
  • From Sigma
• 10mL 50 mM CaCl₂
  • From Emanuele: not necessary for Caulobacter growth at MIT (perhaps enough calcium already in yeast extract or kitchen flasks).
• 1L H₂O (milliQ or distilled)

Instructions

• Add ingredients and autoclave (250°F, 22psi, 30-60min depending on volume).
• For plates, usually add 1.5% (w/v) BactoAgar as well.

Notes

• If PYE is to be aliquoted, first prepare 1L as described, then aliquot/pour into 100mL bottles. Finally, reautoclave individual bottles