Lauber: I won't forget: Difference between revisions
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(New page: * All clones stored in 15% glycerol? * RE digest not working, due to: **dirty prep (high ionic strength) **Organic solvent carry-over (EtOH - unlikely, 30 minutes in dessicator) **Too much...) |
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Revision as of 23:00, 6 September 2009
- All clones stored in 15% glycerol?
- RE digest not working, due to:
- dirty prep (high ionic strength)
- Organic solvent carry-over (EtOH - unlikely, 30 minutes in dessicator)
- Too much DNA
- PEG? - consider using a PEGless minipres
- pvuII fast cutter, consider incubating for 30 minutes only
