Lauber: I won't forget: Difference between revisions
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*Ask Pam about what she does when checking for complete digestion. She transforms cells with her digested sample to check the background before T-tailing, but how? Does she ethanol precipitate, extract from gel and then transform? | *Ask Pam about what she does when checking for complete digestion. She transforms cells with her digested sample to check the background before T-tailing, but how? Does she ethanol precipitate, extract from gel and then transform? | ||
*Check EDTA, is it salt or what? | *Check EDTA, is it salt or what? | ||
http://www.protocol-online.org/biology-forums/posts/4786.html | |||
Latest revision as of 13:12, 15 September 2009
- pvuII fast cutter, consider incubating for 30 minutes only
- Ask Pam about what she does when checking for complete digestion. She transforms cells with her digested sample to check the background before T-tailing, but how? Does she ethanol precipitate, extract from gel and then transform?
- Check EDTA, is it salt or what?
http://www.protocol-online.org/biology-forums/posts/4786.html
