Lauren M. Magee Week 2

Word Definitions

1. permease: Any of several enzymes that transport membrane proteins.(Source:https://glosbe.com/en/en/permease)
2. assimilation: The process of adapting or adjusting to the culture of a group or nation. (Source: http://dictionary.reference.com/browse/assimilation)
3. extracellular: Occurring or being (situated) outside the cell or cells. (Source: http://www.biology-online.org/dictionary/Extracellular)
4. intracellular:Occurring or being (situated) inside a cell or cells. (Source: http://www.biology-online.org/dictionary/Intracellular)
5. flux: Occurring or being (situated) inside a cell or cells. (Source: http://www.biology-online.org/dictionary/Flux)
6. biomass: The total mass of living matter within a given unit of environmental area. (Source: https://dictionary.search.yahoo.com/search;_ylt=A86.J7r6RMdUvVEAFYInnIlQ;_ylu=X3oDMTB0ZWVkYm84BHNlYwNzYwRjb2xvA2dxMQR2dGlkA1lIUzAwMl8x?p=biomass&.sep=)
7. parameters: One of a set of measurable factors that define a system and determine its behavior and are varied in an experiment. (Source: https://dictionary.search.yahoo.com/search;_ylt=AwrTca9CRcdULZUAr70nnIlQ;_ylu=X3oDMTB0ZWVkYm84BHNlYwNzYwRjb2xvA2dxMQR2dGlkA1lIUzAwMl8x?p=parameter&.sep=)
8. cultured: General term referring to the maintenance of cell strains or lines in the laboratory. (Source: http://www.biology-online.org/dictionary/Cell_culture)
9. transferase: A suffix to the name of an enzyme indicating that it transfers a specific grouping from one molecule to another, for example acyl transferases transfer acyl groups. (Source: http://www.biology-online.org/dictionary/Transferase)
10. gram-negative: Of, or relating to the group of bacteria that take the color of the counterstain (i.e. pink) under the microscope following gram staining. (Source: http://www.biology-online.org/dictionary/Gram-negative)

Paper Outline

Introduction

Ammonia is a preferred nitrogen source for Saccharomyces cerevisiae. Nitrogen metabolism is regulated at both the level of gene expression and the level of enzyme activity. Research has pointed out the influence of the concentration of ammonia and the rate of ammonia assimilation. Because of this interaction, the researchers hypothesized that the ammonia flux may have a larger impact than the concentration of ammonia in nitrogen metabolism. To study this, the researchers observed cultures having the same levels of flux but having feeds with different ammonia concentrations.

Material & Methods

S. cerevisiae was used to observe the effects of differing ammonia concentrations gene expression and enzyme activities. S. cerevisiae was continuously cultures and then feed ammonia in concentrations ranging from 29 to 118 mM along with a fixed glucose concentration and dilution rate.

Lauren M. Magee

1. Week 1
   • BIOL398-04/S15:Week 1
   • BIOL398-04/S15:Class Journal Week 1
2. Week 2
   • BIOL398-04/S15:Week 2
   • Lauren M. Magee Week 2
   • BIOL398-04/S15:Class Journal Week 2
3. Week 3
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4. Week 4
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5. Week 5
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6. Week 6
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7. Week 7
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8. Week 8
   • Assignment Cancelled
9. Week 9
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   • Lauren M. Magee Week 9
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10. Week 10
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11. Week 11
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12. Week 12
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13. Week 13
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14. Week 14
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15. Week 15
    • BIOL398-04/S15:Week 15
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