LeBauer:Protocol/Enzyme: Difference between revisions
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*Steven Allison 2008 ([https://webfiles.nacs.uci.edu/allisons/public/index.html Allison Lab]), | *Steven Allison 2008 ([https://webfiles.nacs.uci.edu/allisons/public/index.html Allison Lab]), | ||
**[https://webfiles.nacs.uci.edu/allisons/public/Protocols/ColorimetricEnzymeAssays.pdf Steve's Protocol] | **[https://webfiles.nacs.uci.edu/allisons/public/Protocols/ColorimetricEnzymeAssays.pdf Steve's Protocol] | ||
* | *Bob Sinsabaugh 1994 ([http://biology.unm.edu/sinsabaugh/ Center for Dead Plant Studies]) | ||
==Materials== | ==Materials== |
Revision as of 11:07, 30 April 2008
Overview
Enzyme assays for litter and soil.
Adapted from
- Steven Allison 2008 (Allison Lab),
- Bob Sinsabaugh 1994 (Center for Dead Plant Studies)
Materials
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.
- 1.0 M NaOH
- 4g NaOH pellets
- 100mL DI water
- 50 mM sodium acetate buffer (for 1 L)
- 4.37 g sodium acetate anhydrous (CAS 127-09-3)
- 1.1 ml glacial acetic acid (CAS 64-19-7)
- fill 1000 ml volumetric flask
- titrate solution to pH = 5 with additional acetic acid
- X μL reagent 2
- component A (reagent 2 is made up of multiple components)
- component B
- equipment 1
- equipment 2
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.