Lewis' lab notebook: Difference between revisions
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**5 minipreps ([[R0010]], [[E0241]], [[E7104]]) [[Miniprep/Qiagen_kit|Protocol used]] | **5 minipreps ([[R0010]], [[E0241]], [[E7104]]) [[Miniprep/Qiagen_kit|Protocol used]] | ||
**R0010 and E0241 digests; digests were done according to [[Knight:Restriction_Digest]]; recipe halved. | **R0010 and E0241 digests; digests were done according to [[Knight:Restriction_Digest]]; recipe halved. | ||
**Phosphatase was added to R0010 samples; protocol [[Phosphatase_treatment_of_linearized_vector|here]]. | |||
**Gel run to separate out DNA fragments resulting from R0010 and E0241 digests. 130V, 45 min. | |||
Revision as of 14:29, 14 June 2006
6/14
- Morning:
- 5 minipreps (R0010, E0241, E7104) Protocol used
- R0010 and E0241 digests; digests were done according to Knight:Restriction_Digest; recipe halved.
- Phosphatase was added to R0010 samples; protocol here.
- Gel run to separate out DNA fragments resulting from R0010 and E0241 digests. 130V, 45 min.
