Lewis' lab notebook: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
6/14 | ==6/14== | ||
*5 minipreps ([[R0010]], [[E0241]], [[E7104]]) [[Miniprep/Qiagen_kit|Protocol used]] | |||
*R0010 and E0241 digests; digests were done according to [[Knight:Restriction_Digest]]; recipe halved. | |||
*Phosphatase was added to R0010 samples; protocol [[Phosphatase_treatment_of_linearized_vector|here]]. | |||
*Gel run to separate out DNA fragments resulting from R0010 and E0241 digests. 130V, 45 min. | |||
==6/15== | |||
test | |||
Revision as of 14:37, 14 June 2006
6/14
- 5 minipreps (R0010, E0241, E7104) Protocol used
- R0010 and E0241 digests; digests were done according to Knight:Restriction_Digest; recipe halved.
- Phosphatase was added to R0010 samples; protocol here.
- Gel run to separate out DNA fragments resulting from R0010 and E0241 digests. 130V, 45 min.
6/15
test
