Lewis' lab notebook: Difference between revisions
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==6/15== | ==6/15== | ||
*Carried out ligation reaction with Matt/Tiff's DNA. Protocol according to [[Silver:_Ligation|Silver Ligation]] but with 5 minute incubation. | *Carried out ligation reaction with Matt/Tiff's DNA. Protocol according to [[Silver:_Ligation|Silver Ligation]] but with 5 minute incubation. | ||
*Transformed TOP10 cells. [[TOP10_chemically_competent_cells|( | *Transformed TOP10 cells. [[TOP10_chemically_competent_cells|(Preparing TOP10 cells)]] [[Transforming_chemically_competent_cells|(Transformation)]] | ||
Revision as of 09:34, 15 June 2006
6/14
- 5 minipreps (R0010, E0241, E7104) Protocol used
- R0010 and E0241 digests; digests were done according to Knight:Restriction_Digest; recipe halved.
- Phosphatase was added to R0010 samples; protocol here.
- Gel run to separate out DNA fragments resulting from R0010 and E0241 digests. 130V, 45 min.
- Bands were almost invisible (wells used were those to the right of the second ladder). This may have been due to a second wash step during the mini-preps (although this is unlikely).
6/15
- Carried out ligation reaction with Matt/Tiff's DNA. Protocol according to Silver Ligation but with 5 minute incubation.
- Transformed TOP10 cells. (Preparing TOP10 cells) (Transformation)
