Lewis' lab notebook

6/14

• 5 minipreps (R0010, E0241, E7104) Protocol used
• R0010 and E0241 digests; digests were done according to Knight:Restriction_Digest; recipe halved.
• Phosphatase was added to R0010 samples; protocol here.
• Gel run to separate out DNA fragments resulting from R0010 and E0241 digests. 130V, 45 min.
• Bands were almost invisible (wells used were those to the right of the second ladder). This may have been due to a second wash step during the mini-preps (although this is unlikely).

6/15

• Carried out ligation reaction with Matt/Tiff's DNA. Protocol according to Silver Ligation but with 5 minute incubation.
• Transformed TOP10 cells. (Preparing TOP10 cells) (Transformation)